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Fig. 4. In situ hybridization for XpabpII on whole embryos and guts. (A) A section of early gastrula stage embryo showing XpabpII expression in prospective neuroectoderm. An arrow indicates dorsal blastopore lip. (B) A cross-section of a neurula stage embryo. XpabpII mRNA is strongly expressed in the neural tissue. Inset is the enlarged view of the square part. Two arrowheads indicate neural tube. (C) The lateral view of an early tailbud stage embryo sectioned sagittally. XpabpII is detectable in brain, spinal cord and eye. (D) The dorsolateral view of the head region at stage 28. XpabpII is expressed in whole brain and eye (ey). (E) The dorsolateral view of the head region of a stage 35/36 embryo. XpabpII transcripts are uniformly expressed in the most part of the developing brain vesicles including telencephalon (tel), diencephalon (di) and mesencephalon (me). (F) The head region of a stage 39 embryo viewed dorsolaterally. XpabpII is localized to specific brain region, being strongly expressed in olfactory pit (op), eye (ey), telencephalon (tel), mesencephalon (me) and rhombencephalon (rh) but absent from diencephalon (di). (G) Stage 37 embryonic gut to show XpabpII expression restricted to the anterior gut including liver (li), stomach (st), dorsal (dp) and ventral (vp) pancreatic rudiments. Anterior is to the left. (H) Left view of XpabpII expression in the stage 41 gut in which the pancreatic buds have fused. (I) The ventral view of stage 42 gut. The posterior expression boundary of XpabpII corresponds to the transitional zone/intestine boundary (an arrowhead). (J) The ventral view of stage 44 gut. XpabpII is expressed at a high level in the pancreas (p), but the expression in liver and stomach is much reduced. (K) The pancreatic expression of XpabpII in stage 46/47 gut. Strong signal is only observed in the pancreas, but not in other organs. Inset shows opposite side of pancreas. The positive signals are absent from liver (li), gall bladder (gb) and stomach (st). (L) In situ hybridization in stage 45 gut using sense probe against XpabpII. No positive signal is visible. ey, eye; op, olfactory pit; dp, dorsal pancreatic bud; vp, ventral pancreatic bud; p, pancreas; li, liver; st, stomach; gb, gall bladder; int, intestine; si, small intestine; co, colon; pd, proctodeum. Scale bar: (A–C, G–L), 500 μm; (D–F), 250 μm. |
