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ppardxenopus mesoderm [+] 

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Experiment details for ppard

PPARβ Interprets a Chromatin Signature of Pluripotency to Promote Embryonic Differentiation at Gastrulation.

PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation.

Gene Clone Species Stages Anatomy
ppard.S laevis NF stage 11 mesoderm

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  Figure S1 A dedicated peptide-derived antibody detects endogenous PPARb protein throughout Xenopus laevis early development. (A) Multiple alignments of the protein sequences of xPPARb, xPPARa, and hPPARb/d. The region in blue, which corresponds to the peptide used to generate the xPPARb antibody, is not conserved. (B) DNA affinity purification of gastrula extracts using a 36 peroxisome proliferator-activated receptor response element biotinylated probe in the presence of increasing concentrations of the PPARb agonist GW501516. The lane labelled ‘‘embryo extract’’ corresponds to the input. (C) Immunoblot showing endogenous levels of PPARb protein in total embryo extracts. b-actin is shown as a loading control. Numbers refer to developmental stages. The arrows mark the beginning of the indicated phases. (D)–(L) Immunolocalization of endogenous PPARb protein. Sections of gastrula (stg. 11; D–I) and early tailbud (stg. 31; J–L) processed to immunolocalize endogenous PPARb and observed by fluorescence microscopy are presented. (D) PPARb signal. (F) DAPI signal obtained from the same section. (H) Overlay of the PPARb and DAPI signals. (E), (G), (I), Close- ups of (D) f, and h, respectively, showing nuclear localization. Similarly, (J), (K), and (L) were obtained from the same section and represent the PPARb signal, the DAPI signal, and the overlay of both signals, respectively. Scale bar is 500 mm in (H) and (L) and 100 mm in (I).