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Fig. S3. Mapping of the A/P limit of prdm12 expression domain in the developing neural tube. (A,B) Dorsal view of neurula embryos analysed by double in situ hybridization with a prdm12 antisense probe and with krox20 or mafB probes. All probes are revealed in dark blue. Note that at neurula stages prdm12 is caudal to krox20 and mafB, and that its expression terminates at the domain of mafb expression. (C) Horizontal section at the level of the ventral neural tube of a prdm12 in situ hybridized stage 28 Xenopus embryo. Anterior is to right. The otic vesicle that marks rhombomere 4 is indicated (arrowhead). Note that at that stage, prdm12 expression extends to the anterior hindbrain. |
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Fig. S5. prdm12 expression compared to dbx1, nkx6-1 and nkx6-2 in the posterior hindbrain of Xenopus tailbud stage embryos. (A-H) Transverse sections of the neural tube of stage 28 embryos hybridized with the indicated probes. In the double in situ hybridization experiments shown in E-H, both probes are revealed in dark blue. Note the gap of staining between nkx6-1 or nkx6-2 and dbx1, which corresponds to the p1 progenitor domain (brackets in E, F) and the absence of this gap between nkx6-1 or nkx6-2 and prdm12. |
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Fig.1. Prdm12 expression in Xenopus, chicken and amphioxus embryos. (A-G) Whole-mount ISH analysis of prdm12 expression in Xenopus. In C,E,F, transverse sections at the levels indicated in B,D are shown. (H-M) Double ISH comparing prdm12 with the indicated genes. In panels H,L and M, both Prdm12 and the second probe are revealed in dark blue. In panels I-K, prdm12 is in dark blue and the second probe is in light blue. (N) Diagram summarizing the expression domains of Prdm12 and of HD TFs in the ventricular zone of the Xenopus ventral caudal neural tube. (O) A transverse section of the neural tube of a chick embryo hybridized for Prdm12 and immunostained for Dbx1 (red). (P,Q) Prdm12 and Engrailed are co-expressed in restricted cells of the ventral nerve cord (arrows) of amphioxus larvae (48 hpf). Lateral and dorsal views as well as a transverse section are shown. |
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prdm12 (PR domain containing 12) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 32, partial lateral view, anterior right, dorsal up. |
