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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
prkci	xenopus		mesoderm [+] Eberth-Kastschenko layer,abdominal myoblast,anterior dorsal lateral plate region,anterior notochord,anterior palatine artery,anterior ventral blood island,atrial septum,atrioventricular canal,atrioventricular canal endocardium,atrioventricular valve,atrioventricular valve leaflet,axial ossicle,cardiac atrium,cardiac jelly,cardiac myocyte,cardiac progenitor cell,cardiac valve,cardiac ventricle,caudal presomitic mesoderm,cerebral artery,chordoneural hinge,cloaca,cloacal sphincter,cranial skeleton,dermatome,dermomyotome,distal tubule,dorsal fin vasculature,dorsal lateral plate mesoderm,dorsomedial edge of myotome,early distal tubule,early proximal tubule,endocardial cushion,endocardial tube,endocardium,endothelial cell,enteric neuron,epicardial precursor cell,epicardium,external carotid artery,filter chamber,flexural organ,gamete,glomeral nexus,glomerular epithelial cell,glomerular mesangial cell,glomerulus,glomus,hematopoietic stem cell,involuted dorsal mesoderm,involuted ventral mesoderm,late distal tubule,late proximal tubule,left atrium,mandibular mesenchyme,mesonephric collecting duct,mesonephric tubule,migrating macrophage,myocardium,myocoelic slit,myotome,neurenteric canal,notochordal sheath,ophthalmic artery,outflow tract,pericardial muscle,pericardial roof,pericardium,perichordal tissue,podocyte,postcranial axial skeleton,posterior notochord,posterior palatine artery,posterior ventral blood island,posterior wall of neurenteric canal,prechordal plate,presomitic mesoderm,pronephric duct,pronephric nephron,pronephric nephrostome,pronephric sinus,pronephric tubule,proximal tubule,proximal tubule branch,rectal diverticulum,rib,right atrium,rostral presomitic mesoderm,sclerotomal cartilage,sclerotomal mesenchyme,sclerotome,sinus venosus,smooth muscle myocyte,somatic layer of lateral plate mesoderm,spiral septum,splanchnic layer of lateral plate mesoderm,stratum compactum,stratum spongiosum,syndetome,trabecula carnea,transverse process,urostyle,vacuolated notochordal tissue,ventral blood island border,ventral fin vasculature,ventral mesoderm,ventricular outflow tract,ventrolateral edge of myotome,vertebra,vertebral centrum,vertebral column,vertebral element,Mullerian duct,anterior cardinal vein,anterior lymph sac,anterior palatine artery,aortic arch,arteria iliaca communis,arteria mesenterica posterior,arteria subclavia,arterial endothelium,axial mesoderm,axial skeleton,capillary system of liver,cardiac atrium,cardiac mesoderm,cardinal vein,caudal artery,caudal vein,ceratobranchial I,ceratobranchial II,ceratobranchial III,ceratobranchial IV,cerebral artery,cervical vertebra,chondroblast,chondrocyte,circumblastoporal collar,cloacal sphincter,coeliaco-mesenteric artery,coelomic epithelium,commisura quadratocranialis anterior,cornua trabecula,cranial muscle,cranial muscle primordium,dermatome,dermis,dorsal aorta,dorsal fin vasculature,dorsal lymph space,duct of Cuvier,elastica externa,endocardial tube,endocardium,epicardium,erythroid cell,external carotid artery,external gill,external jugular vein,facies hyoidis of the palatoquadrate,fin vasculature,flexural organ,gastric vein,genital ridge,genital system,gill primordium,glomeral mesenchyme,glomeral nexus,glomus,head mesoderm,head somite 1,head somite 2,head somite 3,head somite 4,heart,heart primordium,hepatic portal vein,hepatic vein,infrarostral,intermediate mesoderm,internal carotid artery,internal jugular vein,interrenal vein,intersomitic artery,intersomitic vein,intersomitic vessel,kidney,larval aorta,lateral plate mesoderm,lateral postcardinal vein,left channel of ventral aorta,left lymph heart,left spleen primordium,lower blastopore lip,lymph vessel,m. cucullaris,m. depressor mandibulae,m. levator scapulae inferior,m. levator scapulae superior,m. petrohyoideus,medial abdominal vein,medial cardinal vein,mesenchymal stem cell,mesonephric duct,mesonephric early distal tubule,mesonephric early proximal tubule,mesonephric late distal tubule,mesonephric late proximal tubule,mesonephric mesenchyme,mesonephric nephron,mesonephric sinus,musculoabdominal vein,myeloid cell,myocardium,myotome of head somite 1,myotome of head somite 2,myotome of head somite 3,myotome of head somite 4,myotome of tail somite 14,myotome of tail somite 15,myotome of tail somite 16,myotome of tail somite 17,myotome of tail somite 18,myotome of trunk somite 1,myotome of trunk somite 10,myotome of trunk somite 11,myotome of trunk somite 12,myotome of trunk somite 13,myotome of trunk somite 2,myotome of trunk somite 3,myotome of trunk somite 4,myotome of trunk somite 5,myotome of trunk somite 6,myotome of trunk somite 7,myotome of trunk somite 8,myotome of trunk somite 9,neurenteric canal,notochord,notochordal epithelium,occipitovertebral artery,omphalomesenteric vein,oviduct,paired dorsal aorta,palatoquadrati,parachordal,paraxial mesoderm,planum hypobranchiale,planum trabeculare anticum,posterior cardinal vein,posterior vena cava,posterior ventral blood island,prechordal plate,presomitic mesoderm,presumptive paraxial mesoderm,primary heart field,processus anterior hyalis,processus articularis palatoquadrati,processus ascendens palatoquadrate,processus lateralis hyalis,processus muscularis palatoquadrati,processus posterior hyalis,processus retroarticularis of Meckel's cartilage,proctodeum,proepicardium,pronephric duct,pronephric kidney,pronephric mesenchyme,pronephric nephrostome,pronephric sinus,pulmonary vein,right channel of ventral aorta,right lymph heart,right spleen primordium,sacral vertebra,sclerotome of head somite 4,sclerotome of tail somite 14,sclerotome of tail somite 15,sclerotome of tail somite 16,sclerotome of tail somite 17,sclerotome of tail somite 18,sclerotome of trunk somite 1,sclerotome of trunk somite 10,sclerotome of trunk somite 11,sclerotome of trunk somite 12,sclerotome of trunk somite 13,sclerotome of trunk somite 2,sclerotome of trunk somite 3,sclerotome of trunk somite 4,sclerotome of trunk somite 5,sclerotome of trunk somite 6,sclerotome of trunk somite 7,sclerotome of trunk somite 8,sclerotome of trunk somite 9,secondary heart field,smooth muscle,somatic layer of lateral plate mesoderm,somite,spiral septum,splanchnic layer of lateral plate mesoderm,spleen,subinterstitial vein,subocular cartilage,suprarostral plate,tail bud,tail somite 14,tail somite 15,tail somite 16,tail somite 17,tail somite 18,tail vein,trabecula carnea,trabecula cranii,trunk somite 1,trunk somite 10,trunk somite 11,trunk somite 12,trunk somite 13,trunk somite 2,trunk somite 3,trunk somite 4,trunk somite 5,trunk somite 6,trunk somite 7,trunk somite 8,trunk somite 9,urostyle,vascular endothelium,venous endothelium,ventral aorta,ventral blood island,ventral blood island border,ventral fin vasculature

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Experiment details for prkci

Spatiotemporally Controlled Mechanical Cues Drive Progenitor Mesenchymal-to-Epithelial Transition Enabling Proper Heart Form...

Gene	Clone	Species	Stages	Anatomy
prkci.L		laevis	NF stage 15 to NF stage 32	endocardial tube

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Figure 1. (A) Xenopus embryos at three stages during HPC (red) movement to the ventral midline. hpf, hours post-fertilization. (B) Transverse schematic of HPCs and their microenvironment (blue, ectoderm; red, mesoderm; yellow, endoderm; brown, fibronectin; purple, fibrillin; orange, aPKC; green, ZO-1; dark red, tropomyosin). (C) Cell shapes by β-catenin localization and cardiomyocytes by tropomyosin expression. Note: red spot (asterisk) in stage 28 is non-specific staining outside the embryo. (D) aPKC appears on the apical surface of HPCs (white arrows) with fibrillin at the HPC basal surface. (E) Tight junction protein ZO-1 appears perinuclearly and nascently on the apical surface by stage 23 and strongly at HPC apical intercellular junctions by stage 28. Fibronectin appears at all germ-layer interfaces. (F–I) Apical aPKC (F and G) and ZO-1 (H and I) during early heart-tube formation. Immunofluorescence shows epithelial individual markers and tropomyosin (F and H, upper panels) and normalized intensity with pseudocolor lookup table (LUT) (F and H, lower panels). (G and I) Quantification of apical polarity from five or six embryos per time point over two clutches. Scale bars, 50 μm. ∗p < 0.05, ∗∗p < 0.01. See also Figure S1.

Gene	Clone	Species	Stages	Anatomy
prkci.L		laevis	NF stage 28	primary heart field , cardiac myocyte , mesoderm

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Figure 2. (A–C) Modulators of tissue compliance applied during stages of early heart development exhibit defects, including pericardial and neural edemas (A) (see arrows; scale bar, 1 mm), altered AP length (B), and increased rates of edema per clutch (C) (n = 30–35 embryos over four clutches). (D) Compliance measured by microaspiration of HFR. (E) Compliance at stage 22 confirms that blebbistatin and Y27632 increase and calyculin A decreases compliance (n = 11–17 embryos per treatment over three clutches). (F) Transverse sections through HFR at stage 28 show changes in polarity (aPKC or ZO-1) within the progenitor population (red). Lower panels show the epithelial marker masked using tropomyosin expression (scale bar, 50 μm). (G) Apical intensity after small-molecule inhibitor treatment (n = 9–13 embryos over four clutches). (H) Representative lateral confocal sections of stage 39 tadpole hearts (scale bar, 100 μm). (I) Cardiac anatomy after stage-specific inhibitor treatments as shown in Figure S2 (n = 5 embryos per treatment per period). Error bars represent mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. See also Figures S1, S2, and S3A.