| |
Fig. 4. Transcript encoding the 43K protein and
transcript encoding the AChR alpha subunit are 25- to
35-fold more abundant in denervated than in innervated
adult muscle. The triceps femoris muscle of adult
Xenopus was denervated for 10 days and AChR alpha
subunit and 43K protein transcript levels were measured
by RNase protection. 5 fig of RNA from innervated
(INN) and denervated (DEN) muscle were included in
hybridizations with 32P-labelled probes. The amount of
alpha subunit and 43K protein transcript is low in
innervated muscle and increases 25- to 35-fold in
denervated muscle. Denervation results in no change in
either the amount of total RNA or the level of transcript
encoding the translation elongation factor Ef-1-alpha
(Baldwin et al. 1988). The arrowheads mark the positions
of the protected fragments at 403 nucleotides (43K
protein) and 429 nucleotides (AChR alpha subunit). |
