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Figure 1. Hermes/Rbpms protein and nanos RNA localize within germinal granules unique to germ plasm. Stage I oocyte. (A) Immunofluorescence (IF) and confocal microscopy showing Hermes/Rbpms protein (green) is ubiquitous in stage I oocyte including the mitochondrial cloud (MC), nucleus (N) and ooplasm; microtubules (red). Enlarged image from (A) showing microtubule organization around MC (B) and Hermes/Rbpms (C); Note how Hermes/Rbpms is enriched within the germ plasm region of the MC; (D) Electron micrograph showing a region of germ plasm within the MC of stage I oocyte. Red arrows indicate two germinal granules; (E) Nanos RNA is localized within germinal granules in stage I oocytes as is Hermes/Rbpms protein (F). Electron microscopy in situ hybridization with antisense Digoxigenin labeled nanos RNA was visualized with nanogold conjugated anti-Dig antibody and silver enhancement. Electron microscopy immunostaining with Hermes/Rbpms polyclonal antibody [49], anti-rabbit nanogold conjugated secondary antibody and silver enhancement. Scale bars are as indicated. GG (germinal granules); M (mitochondria); N (nucleus). |
