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Fig. 9. Developmental expression pattern of Xrgs4a.
(A-H) Spatial expression pattern of Xrgs4a. Whole-mount
in situ hybridization analysis of gene expression in albino
embryos at the gastrula, neurula and tailbud stages.
(A,C,E,G) In situ hybridizations were performed with an
antisense Xrgs4a-probe. (B,D,F,H) In situ hybridizations
were performed with a sense Xrgs4a-probe. (A,B) Vegetal
view of stage-10.25 embryos. (C-H) Lateral view of stage-
16 (C,D), stage-25 (E,F) and stage-35 (G,H) embryos.
Anterior is right. Xrgs4a is expressed in the dorsal lip of
the blastopore, with expression extending over the dorsal
aspect of the gastula-stage embryo (A). Xrgs4a is
expressed in the neural folds of neurula-stage embryos
(C,E). Xrgs4a is expressed in the head, retina, spinal cord,
cement gland and heart of the tailbud-stage embryo (G). (I) Timing
of Xrgs4a gene expression. RNA was purified from whole embryos
at the indicated embryonic stages (Nieuwkoop and Faber, 1967). RTPCR
was performed by use of specific primers that recognized
Xrgs4a or ornithine decarboxylase (ODC), as described in Materials
and Methods. The 40â RT lane contained all reagents except reverse
transcriptase, and was used as a negative control; ODC was used as a
loading control. |
