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Fig. 2. Spatial expression pattern of xPN-1 in embryogenesis. (A) RT-PCR of the animal region (A), the marginal region (M), the vegetal region (V), the mixture of the dissected three parts (+++) and intact whole embryos (W) at Stage 9. (B–Q) in situ hybridization of xPN-1. (B) Stage 13 in dorsal view. The anterior side is up. (C) A transverse section of a Stage 13 embryo. (D) Stage 15 in dorsal view. The anterior side is up. (E) Transverse section of a Stage 20 embryo. (F–H) Stage 25. Red lines in F indicate the planes of the section shown in H and I. The embryo was cleared in G. (J–M) Stage 30. Red lines in J indicate the planes of the section shown in L and M. The embryo was cleared in K. (N–Q) Stage 35. Red lines in N and P indicate the planes of the section shown in P and Q, respectively. The embryo was cleared in O. br, brain; no, notochord. np, neural plate; ov, otic vesicle; ph, pharynx; pe, pharynx endoderm. |
