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FIGURE 1 | Expression of early markers of the diencephalic
prepatterning and formation of the Zli. Microphotographs of lateral
views of the forebrain in whole mounts labeled for double in situ
hybridization (ISH) to reveal Shh (purple) and Gbx2 (orange) at the
embryonic stages indicated (A–D); the orientation is indicated in each
panel to highlight the dorsoventral and rostrocaudal change of the axis
through the thalamus at each stage. (E–O): Microphotographs of whole
mounts (I,J,M) and sagittal (E,F,H,K,L,N) or transverse (G,O) sections of
embryos at stages 33/34. The photographs correspond to single ISH
(E,I,J), single immunohistochemistry (IHC; K), and combinations of
double ISH (F,G,M–O) or combined ISH and IHC (H,L). The markers
labeled are indicated in the upper left of each photograph. All images
are oriented following the same standard: dorsal is upwards in transverse and sagittal sections, and rostral is to the left in sagittal sections. The neuromeric boundaries and main brain subdivisions are indicated to assist in the precise localization of the labeling. The levels of the transverse sections (G,O) are indicated in photographs (F,N), respectively. Scale bars = 100 µm (E,F,L–N), 50 µm (A–D,G–K,O). See list for abbreviations.
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