Search Criteria |
Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
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smad1 | xenopus | somite [+] |
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Experiment details for smad1
Ho DM and Whitman M (2008) AssayTGF-beta signaling is required for multiple processes during Xenopus tail regeneration.
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Fig. 7. Early inhibition of TGF-β signaling prevents subsequent activation of the BMP signaling pathway in the regeneration bud. Regenerates treated with 100 μM SB-431542 or 0.2% DMSO from 0 to 8 hpa were harvested at 2 dpa and processed for the following markers: (A) immunofluorescence against phospho-Smad1 (p-Smad1, green), showing that p-Smad1 in the regeneration bud proper is eliminated by SB-431542 treatment. p-Smad1 (blue) in unamputated stage-matched tadpole tails was unaffected by SB-431542 (bottom row); (B) in situ hybridization with xMsx1 antisense probe (purple)—black arrow: xMsx1 expression in the dorsal blastema; (C) immunofluorescence for the mitotic marker phospho-Histone H3 (pH3, green). Total DNA in panels A and C is shown in blue. Arrowheads: amputation plane. Scale bar: 100 μM. |