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Fig. 2. p-Smad2 immunostaining at later tailbud stages. (A) Tailbud-stage embryos were treated with 100 μM SB-431542 or 0.2% DMSO for 22 h prior to harvesting at stage 29 or 36. Embryos were stained with anti-p-Smad2 and cleared. (B) Stage 34 embryos stained for p-Smad2 were cleared to reveal specific structures in the head (top) and tail (bottom). Note that some bilateral structures can be seen twice in cleared heads. br, brain; e, eye; ov, otic vesicle; ba, branchial arches; m, mouth; tb, tailbud; f, fin; nt, posterior neural tube; pw, posterior wall of tailbud; cnh, chordoneural hinge. |
