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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
sox2	xenopus		pallium

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Experiment details for sox2

Moreno N and González A (2017) Assay

Gene	Clone	Species	Stages	Anatomy
sox2.S		laevis	NF stage 37 and 38	pallium

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FIGURE 6. Analysis of progenitor cells at embryonic stages (BLBP, Lhx2, Sox2, Pax6, PH3). Micrographs of transverse sections through the pallium of Xenopus laevis at embryonic stages showing the codistribution of the progenitor markers BLBP (A,C), Lhx2 (B,C,E–G), Sox2 (D), and Pax6 (E,F′); and the combination of Lhx2 with the mitotic marker PH3 (G,H). In each panel the developmental stage and the color code for the used markers are indicated. (F,F′) Are confocal images, and the higher magnification shown in (F’) corresponds to the framed area indicated in (F). The BLBP (A) and the Lhx2 (B) labeling detected at stage 37/38 showed double labeled cells exclusively in the ventricle (C). The combination of Lhx2 and Sox2 also showed double labeled cells in the ventricle, but the cells detected for both markers in the mantle were never double labeled (D). In the case of Pax6, Lhx2/Pax6 double labeled cells were observed in the ventricular zone (E,F′), but never away from this region, where both cell populations were intermingled (F′). The labeling of Lhx2 in combination with PH3 showed that only the ventricular cells expressing Lhx2 were mitotic cells (G,H). Scale bars = 50 μm. See list for abbreviations.

Gene	Clone	Species	Stages	Anatomy
sox2.S		laevis	NF stage 37 and 38 to NF stage 42	pallium

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FIGURE 7. Analysis of progenitor cells at embryonic stages (Sox2, DCX, PH3). Micrographs of transverse sections through the pallium of Xenopus laevis at embryonic stages 37/38 (A–D,I,L) and 42 (E–H,J,K,M,N) showing the codistribution of the progenitor marker Sox2 (A,E) and the neuroblast marker DCX (B,F) in combination to DAPI (D,H), and the combination of DCX with the mitotic marker PH3 (I–K). In each panel the developmental stage and the color code for the used markers are indicated. All micrographs are confocal images, and the higher magnifications (L,M,N) correspond to the framed areas, as indicated (white boxes in C,G,K). At the ventricular zone there were observed double labeled cells for Sox2 and DCX (see arrowheads in A–C, E–G), and those cells were mitotic cells (see white circles and filled arrowheads in D,H). Additional double labeled cells were observed away from the ventricle (see empty arrowheads in E–H). The combination of DCX and PH3 showed double DCX/PH3 labeled cells in the ventricle and away from it (see arrowheads in I,J). Scale bars = 25 μm (A–H,L,M), 50 μm (I–K). See list for abbreviations.

Gene	Clone	Species	Stages	Anatomy
sox2.S		laevis	NF stage 46 to NF stage 54	pallium

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FIGURE 9. Analysis of progenitor cells at larval stages (Sox2, DCX, BLBP, PCNA, PH3). Micrographs of transverse sections through the pallium of Xenopus laevis at larval stages 46 (A–E,M) and 54 (F–L,N–R) showing the distribution of the progenitors markers Sox2 (A,C,F,H,I,J,L,N), the neuroblasts marker DCX (B,C,E,G–I,Q) and the progenitors markers Lhx2 (M,N,Q) and BLBP (O,P), and their combinations with the mitotic marker PCNA (L,O,P) and PH3 (M). In addition, the codistribution of DCX and calretinin is shown (Q). In each panel the developmental stage and the color code for the used markers are indicated. (A–E,O,P) Are confocal images, and the higher magnifications (O′,P′,R′) correspond to the framed areas, as indicated (white boxes in O,P,R). At stage 46 Sox2/DCX double labeled cells were observed in the ventricular zone and away from it (arrowheads in A–C), and DCX labeled mitotic cells away from the ventricle were observed (arrowheads in E). At stage 54 Sox2 is expressed in the ventricular and mantle zones, and cells that coexpressed DCX were observed (F–I). Close to the ventricle those Sox2 cells were mitotic cells (J,K), but the Sox2 expressing cells detected away from the ventricle were not mitotic (empty arrowhead in L). The Lhx2 expressing cells detected at larval stages were mitotic in the ventricle (M) and coexpressed Sox2 (N), but in the mantle zone they were not coexpressing PH3 (M) and were intermingled with the Lhx2 cell population (empty arowheads in N). The combination of BLBP and PCNA showed that the mitotic cells in the ventricular zone coexpressed BLBP (arrowheads in O′,P,P′), but not those separated from the ventricle (empty arrowhead in P′). The combination of Lhx2 and DCX showed that the Lhx2 cells detected close to the adjacent vz were double labeled (Q). The combination of Sox2 to calretinin, a marker of a population of pallial interneurons, showed that there were not double labeled cells (R,R′). Scale bars = 50 μm (A–E,L,M,O–P′), 200 μm (I), 100 μm (F–K,N). See list for abbreviations.