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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
twist1
|
|
tropicalis
|
NF stage 22
|
mandibular crest
,
hyoid crest
,
branchial crest
,
neuroectoderm
,
cranial neural crest
|
twist1
|
|
tropicalis
|
NF stage 29 and 30
|
mandibular crest
,
hyoid crest
,
lateral plate mesoderm
,
cranial neural crest
,
anterior branchial crest
,
[+]
|
sox8
|
|
tropicalis
|
NF stage 22
|
mandibular crest
,
hyoid crest
,
branchial crest
,
neuroectoderm
,
endoderm
,
[+]
|
hoxb3
|
|
tropicalis
|
NF stage 22
|
hindbrain
,
neuroectoderm
,
cranial neural crest
,
neural tube
,
rhombomere R5
|
hoxb3
|
|
tropicalis
|
NF stage 29 and 30
|
hindbrain
,
neuroectoderm
,
neural crest
,
cranial neural crest
,
neural tube
,
[+]
|
snai2
|
|
tropicalis
|
NF stage 22
|
mandibular crest
,
hyoid crest
,
branchial crest
,
neuroectoderm
,
posterior neural tube
,
[+]
|
snai2
|
|
tropicalis
|
NF stage 29 and 30
|
mandibular crest
,
hyoid crest
,
chordoneural hinge
,
cranial neural crest
,
neural tube
,
[+]
|
neurod1
|
|
tropicalis
|
NF stage 29 and 30
|
olfactory placode
,
neuroectoderm
,
pineal gland
,
trigeminal placode
,
eye primordium
,
[+]
|
neurod1
|
|
tropicalis
|
NF stage 22
|
neuroectoderm
,
trigeminal placode
,
eye primordium
,
lens placode
,
lateral line placode
,
[+]
|
|
|
Fig. 4. XtSulf2 overexpression disrupts cranial neural crest migration. One blastomere of a two-cell X. tropicalis embryo was injected with 0.5 ng of XtSulf2 mRNA, embryos were allowed to develop to NF stage 22 and 30 and fixed for in situ hybridisation using HoxB3 (A–E), NeuroD (F–I), Slug (J–M), Sox8 (N–Q) and Twist (R–U). (A–B, F–G, J–K, N–O, R–S) Lateral views of control and injected sides of NF stage 22 embryos. (E) Dorsal view of embryo in (A–B), black asterisk indicated the injected side. (C–D, H–I, L–M, P–Q, T–U) Lateral views of control and injected sides of NF stage 30 embryos. At stage 22, on the control side 6–12% of embryos show disrupted migration, while on the injected side 58–71% of migration is found to be inhibited (n = 55–84) as assayed by the expression of CNC markers shown. At stage 30, on the control side 4–5% of embryos show disrupted migration, while on the injected side 40–53% of migration is found to be inhibited (n = 34–81). |