| Search Criteria |
| Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
|---|---|---|---|---|
| tcf7l2 | xenopus | epidermis [+] |
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Experiment details for tcf7l2
Adil MT et al. (2019) Assay
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| Fig. 5. Tcf7l2 immunostaining showing appearance of Tcf7l2 expressing skin cells in the cornea region following whole cornea PUV treatments. (A–D) Normal skin tissue obtained from the flank region of a representative tadpole. (A) DIC image showing pigment cells, which are a characteristic of skin tissue. (B) Skin tissue shows positive immunostaining for Tcf7l2, which labels a subset of apical epithelial skin cells that are not normally present in the cornea. (C, D) are the corresponding Hoechst and merged images for (A, B), respectively. (E-H′) Control and PUV treated corneas obtained from the left and right sides of the same tadpole, respectively. (E, F) shows the peripheral epidermis region (skin that surrounds the cornea), where Tcf7l2 staining is normally observed. (E′, F′) shows the cornea region of the control (untreated) cornea, where no Tcf7l2 staining is normally observed. (G, H) is the peripheral skin region, and (G′, H′) is the cornea region overlying the eye and of a PUV treated cornea collected at 12dpt. The presence of Tcf7l2 expressing cells in the cornea region shows the presence of these skin cells. Note that these Tcf7l2 positive cells appear smaller and more compacted than those found in the surrounding skin. (F, F′, H, H′) are the corresponding merged (Tcf7l2+Hoechst) images of (E, E′, G, G′), respectively. Scale bar in D equals 100 μm and applies to A-D and equals 40 μm in H′ and applies to E-H’. |