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Fig. 6. In situ localization of ID14 mRNA to the epithelia of tadpole skin and tail. Cross-sections of back skin (stage 66) and tail (stage 55) were hybridized with the antisense (A and B) or sense (C and D) RNA probe. Specific signal in the epithelium is seen in sections hybridized with the antisense probe in the skin (compare A and C) and tail (compare B and D). The specific signal in the secretory glands in A is consistent with the epithelial origin of the glands. The dark spots in B–D are melanin pigment granules. e, epithelia; sg, epithelial secretory glands; cl, collagen lamella. Bars, 20 mm. |
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Fig. 5. In situ localization of ID14 mRNA to the intestinal epithelium. Cross-sections of the intestine were hybridized with the antisense (A–D) or sense (E) RNA probes. (A) No specific signals were detected in the larval epithelium (le) at stage 54. (B) No signals were seen in dying larval cells (le) or proliferating, undifferentiated adult epithelial cells (ae) at stages 61 and 62. Identification of the area of adult cell proliferation demarcated by dark line is based on previous studies (Ishizuya-Oka and Ueda, 1996). (C) Signals became detectable in the adult epithelium (ae) at stage 63, and intensified by stage 66 (D). (E) Background signal was weakly seen for the intestine at stage 66 hybridized with the sense probe. ct, connective tissue; m, muscles. Bars, 20 μm. |
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Fig. 5. Tecta.2 expression at stg 66 in intestinal epithelium |
