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Fig. 1. Upregulation by T 3 of TR mRNA in different tissues of premetamorphic Xenopus tadpoles. The figure shows dark-field imaging of
localization by in situ hybridization of 35S-labelled antisense Xenoptts TRa cRNA probe (Kawahara et al., 1991) in saggital sections of brain
(a,b), intestine (c,d) and liver (e,f) of stage 52 tadpoles. (a,c,e) Control (untreated) tadpoles. (b,d,f) Tadpoles treated for 4 days with 10-9M T 3.
Sense probe gave virtually no hybridization signal and the autoradiographs are thus not shown. Arrows in liver sections (e,f) indicate an artefact
produced in black-and-white photography by pigmentation (Pi) surrounding the bulk of the parenchymal cells (Pa) of the liver. The sections were
exposed for one week for autoradiography. Other details as in Kawahara et al. (1991). Bars represent 100 um. |
