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Fig. 4. (A-D) LacZ expression driven by W5aE. Zygotes were injected with W5aELacZ and embryos were cultured until stage 12.5 (late gastrula) and stained for LacZ. Of 102 embryos treated, 92 showed an almost identical expression pattern to the one shown. (A) Dorsal view anterior to left. (B) Anterior view. (C) Section through lateral edge of embryo to reveal staining in the outer ectodermal cells but not other cell layers. (D) Five fold magnification of top left corner of (C). (E-F) In situ hybridisation for XWnt-5a on untreated embryos at stage 12.5. E, dorsal view anterior to left. (F) Anterior view. (G) Section through lateral edge of embryo to reveal staining in the outer ectodermal cells but not other cell layers. (H) Five-fold magnification of top left corner of (G). (I,J) In situ hybridisation for Xotx2 on untreated embryos at stage 12.5. (I) Dorsal view anterior to left. (J) Anterior view. (K) LacZ staining of a stage 12.5 embryo injected zygotically with W5aE-Delta-OTXLacZ, anterior view (62 of the 74 injected embryos showed this pattern). (L) LacZ staining of a stage 12.5 embryo injected zygotically with the LacZ reporter construct containing only the basal promoter element. |
