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Fig. 1. Rogue cells express Gsc, Xwnt8 and Sox17α in the Xenopus early gastrula. In situ hybridization using Gsc, Xwnt8 or Sox17α antisense probes on bisected embryos at early gastrula stage 10-10.5. (A-C) The majority of Gsc expression is seen in dorsal mesendoderm; however, some expressing cells (arrows) are seen in the ventral marginal zone (B′) and the vegetal mass (A′-C′). (D-F) Xwnt8 expression is seen in the ventral marginal zone, although some expressing cells are seen in the dorsal marginal zone (D′) and in the vegetal mass (E′,F′). (G-I) Sox17α expression is strongest in the vegetal mass; however, discontinuous expression is also seen in the marginal zone (G′-I′). Dorsal is towards the left, animal towards the top. (A,D,E,G,H,I) The outside surface of embryo; (B,C,F) the cut surface of embryo. (A′-I′) Higher magnification of the cells indicated in A-I. |
