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Fig. 1. Expression of the
XAWP1 gene. (A) RT-PCR
analysis showing the temporal
expression pattern of AWP1 in
Xenopus early development.
Ornithine decarboxylase (ODC)
serves as a loading control. –RT,
a stage 27 control embryo in the
absence of reverse transcriptase.
(B-I) Spatial expression
pattern of XAWP1. (B) Animallateral
view with the vegetal pole
to the bottom. (C) Vegetal-lateral
view with dorsal to the right.
(D) Sagittal section of a stage
10.5 gastrulae. Arrows in (C,D)
denote the dorsal blastopore
lip. Arrowheads indicate the
involuting dorsal mesoderm.
(E) Dorsal view with anterior to
the top. (F) Anterior view of the
embryo shown in (E) with dorsal
to the top. ne, neural ectoderm;
pe, preplacodal ectoderm. (G,H)
Transverse sections of the embryo in (E) at the levels indicated by the dashed lines. Arrows in (G) denote the preplacodal ectoderm. SM, somitic mesoderm;
nc, notochord; ov, otic vesicle; ba, branchial arch; kt, kidney tubule; sm, somites. (J) Four-cell stage embryos were injected in the animal pole
region as indicated with FGF8a (1 ng), XWnt8 (400 pg) and Xnr-1 (100 pg) and then animal caps were excised at stage 9 and cultured to stage 10.5 for
RT-PCR analysis. WE, a stage 10.5 whole embryo. Con. AC, uninjected control animal caps. |
