XB-CLONE-12441154
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Clone Name: wnt11-409
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Gene:
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Species: Xenopus laevis
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Description: EXRC Clone Number 409.
Xenopus laevis wingless-type MMTV integration site family, member 11B (wnt11b) - For RNA injection.
A dominant-negative construct, lacking the C-terminal portion of the protein, made according to G&D 10: 2805-2817 (1996)
PCR was carried out from pGEM-X9 aka 10.1.4 (Xwnt11 supplied by Doug Melton)as a template. The PCR product obtained was cloned into the XhoI and BglII sites of pSP64TXB.
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Sequence:
Full length sequence: pSP64T-dn-Xwnt11_FullLength
[+]
agctngAataACGCTCAnCTTTGGCaGaTCCTCGAGTACCAATGGCTCCGACCcgtCAcTgGGTTACCCCCctgCTCCTCTTGTGTTGCTCCGGGATCTG
TGGCGCCATCCAGTGGCtTGGGCTCACCGTAAATGGCAGCCGGGTGGCCTGGAATGAGAGCGAACACTGTCGCTTGCTGGACGGTCTAGTCCCTGACCAG
TCGCAACTCTGCAAGAGGAATCTTGAACTGATGCAAAGTGTCGTTAATGCTGCCAAACAGACCAAGCTAACCTGCCAAATGACCTTATCTGACATGCGCT
GGAACTGCTCTTCAGTAGAGAATGCACCAAGCTTCACCCCAGACTTGAGCAAAGGTACCAGGGAGTCTGCCTTTGTCTATGCTCTGGCCTCTGCCACTCT
CAGCCATACCATTGCGCGTGCCTGTGCCTCGGGGGAGCTGCCCACATGCTCTTGTGGTGCCACCCCAGCTGAGGTGCCCGGAACAGGCTTCCGATGGGGA
GGGTGTGGGGACAACCTACATTACGGCCTTAACATGGGCTCTGCTTTTGTTGACGCTCCAATGAAGTCAAGCAAGTCTGCTGGGACCCAGGCCACTAAAA
TTATGAATCTACACAACAATGCAGTTGGCAGACAGGTACTGATGGACTCTTTGGAGACAAAATGCAAGTGCCACGGAGTGTCTGGATCTTGCTCCGTGAA
GACCTGTTGGAAGGGCCTGCAGGATCTTCCCCATATTGCTAATGAGCTTAAATCCAAATACCTTGGTGCCACTAAAGTCATCCACAGACAGACCGGCACT
CGGAGGCAGCATGTTCCCAGGGAGCTAGACATCAGGCCAGTGAGAGAGTCTGAGCTAGTGTACCTGGTTAGTTCCCCTGACTACTGCTGAAAGATCTGGT
TACCACTAAACCAGCCTCAAgaACACCCGAATGGAGTCTCTAAGCTACATAATACCAACTTACACTTTAcaAAATGTTGTcCCCCaAAATGTAGCcatTc
nnATCTGCTCCTAatAAAAAanaAagtTTctTCacaTTcTAAAAAAAAAAAAAannaaaaAAAAAaaAAAAAaCCCCCCCCCCcccCCCCcnngcag
Construction:
Insert:
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| Size: | Source Tissue: | Stage: |
Vector:| Vector Details | Vector Map |
Name: pSP64T
Type: plasmid
5' Restriction Site: null
3' Restriction Site: null |  |
Usage:
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RNA injection:
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| sense |
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SP6 |
BamHI |
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Suppliers:
Search for clone at:
EXRC
