XB-CLONE-1753811
Clone Name: XL435i18ex
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Gene:
rdd2.L
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Species: Xenopus laevis
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Sequence:
5' EST: BP680681 [+]
attttttcagtggattgtatgtccagcttccaactcgtgcctcagaggaaatacactgacaacttcaaaacttggtgaaattcaagatggaattctggaa
caagtattcctggacaaacctgttggtgctggctctgattttcgtgactgttgatcggattcagtctgacgactcaatgtgtccacagggcatggtatac
ggctgcaagcggatttgctacagtaactgtgacaatctaaacagcaccattgaaggctgcattgagatatgtaagctggggtgcgactgcaaagaaggct
tcgtcttccaatccaaaaactccaacacctgcgtccgcccctcttcttgtaaagtctcctgccctaaacacatgacattcaaaccctgcaacaggtttta
tcgcaagacttgttctaatagaaacgtaaaaatggtgccctcggaagtctgcatgccccgctgtgtttgcaatgacggctacatcctctccgatgctcga
cgttgtatcaaagtcaaccagtgcccttaagtgaattaaatcatctgtcccttttaaccggcctatttcctgaaaatgaataaaatcaacattcatagag
tacgagccaataaatgtcatgaacacttctgtcaactccatctgcaaatagagataattggctgcagttgcacagttgtattgttatattattacctata
ttattagtccgcttaatgggattaatatttaatcttctgtatcaaactcagtaaatattagaaaaactgttacttgtgaatatagtgacttcacaatgga
ggaaagtgtaaatattacaaattaacattttt
3' EST: BP717268 [+]tctatatactataatttatccttttaactttgccgactgcataggaggttataaatgacccctcatatctgattcactcacagcacatgggactcagttc
ccaaaaactgctattggcaacccttagtaccctccaatttgagcctgttacccaaccacttagaaggtaagctccgtaatatatggacttatatgtttac
cttttctatagaaacataaaaaaggaaatcaaaaatgttaattgtaatatttacactttcctccattgtgaagtcactatattcacaagtaacagttttc
taatatttactgagtttgatacagaagattaaatattaatcccattaagcggactaataatataggtaataatataacaatacaactgtgcaactgcagc
caattatctctatttgcagatggagttgacagaagtgttcatgacatttattggctcgtactctatgaatgttgattttattcattttcaggaaataggc
cggttaaaagggacagatgatttaattcacttaagggcactggttgactttgatacaacgtcgagcatcggagaggatgtagccgtcattgcaaacacag
cggggcatgcagacttccgagggcaccatttttacgtttctattagaacaagtcttgcgataaaacctgttgcagggtttgaatgtcatgtgtttagggc
aggagactttacaagaagaggggcggacgcaggtgttggagtttttggattggaagacgaagccttctttgcagtcgcaccccngccttacatatctcca
atgcagcccttcnatgggtgctgnt
Library:
Description: About 500 pieces of anterior neural plates were dissected from stage 12-12.5 embryos. The neuroectoderm layer was separated from the underlying mesendoderm layer in 1 x modified Barth's solution containing 1-2 mg/ml collagenase. After poly (A)+RNA selection by an oligo(dT) cellulose column, a cDNA library was made with the SuperScript plasmid system for cDNA synthesis and plasmid cloning (Invitrogen) and the pCS105 vector (Hsu D.R. et al., Mol Cell. 1, 673-83, 1998). The first strand cDNA was synthesized with oligo dT-NotI primer. After second strand synthesis, SalI adapter (gtcgacCCACGCGTCCG; the lower case letters show SalI site) were ligated and the cDNA was directionally cloned with SalI at the 5' end and NotI at the 3' end. The average insert size is about 2kb and the host bacteria is XL1 blue (Stratagene) (?).Vector Details | Vector Map |
Name: pCS105
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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