Summary Attributions

Clone Name: tubb2b-733	Gene: tubb2b.S	Species: Xenopus laevis
Description: EXRC Clone Number 733. Xenopus laevis tubulin, beta 2B class IIb. Image EST 5571885, full length cDNA. Accession AAH44030. To generate probe for in situ.

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Sequence:

Full length sequence: BC044030 [+]

GTTCTTAGCCGGCTGGAAACCATCGGGGTGTTGCACAAAGGGCAAGTAAAGCAACAAGAAAGGGCCACACCATCCACTGATTACACCACTGTAAGACTCA
ACCCATCGGAAACATGCGTGAAATCGTGCACCTCCAAGCTGGCCAGTGCGGTAACCAAATTGGAGCTAAATTTTGGGAAGTCATCAGTGATGAACACGGC
ATTGATCCTACCGGCAGTTACCATGGAGACAGTGATTTGCAACTAGAAAGGATTAACGTATACTACAATGAAGCCACAGGTAACAAATTTGTACCCCGTG
CCATCCTTGTGGATTTGGAACCAGGCACAATGGACTCTGTCAGATCTGGACCATTCGGGCAGATTTTCAGACCAGACAACTTTGTGTTTGGTCAAAGTGG
TGCTGGCAATAACTGGGCCAAAGGTCATTACACCGAAGGAGCTGAGCTGGTGGACTCTGTTCTAGATGTGGTGAGAAAAGAATCTGAGAGCTGTGACTGC
CTACAAGGTTTTCAACTGACCCATTCTCTGGGTGGTGGCACAGGCTCTGGTATGGGTACCCTGCTCATCAGTAAGATAAGGGAAGAGTACCCAGACCGAA
TCATGAATACATTCAGTGTGATGCCATCACCCAAAGTCTCAGACACTGTGGTTGAACCATATAATGCAACCCTCTCTGTTCATCAGTTGGTGGAAAATAC
AGATGAAACCTACTGCATAGACAATGAGGCCCTCTATGATATCTGCTTCCGCACTTTAAAGTTAACAACACCAACATATGGTGATCTGAATCACCTTGTA
TCCGCTACAATGAGCGGGGTAACAACCTGCCTTCGTTTCCCAGGGCAGCTTAATGCTGATCTACGCAAACTGGCTGTCAACATGGTGCCTTTCCCTCGAT
TGCACTTTTTTATGCCAGGCTTTGCCCCATTAACAAGTCGTGGCAGCCAACAATACCGAGCCCTGACAGTGCCAGAACTAACACAGCAAATGTTTGATTC
CAAGAACATGATGGCAGCGTGCGATCCCCGTCATGGACGCTACCTCACAGTAGCTGCTATCTTCCGTGGAAGAATGTCTATGAAGGAGGTAGATGAACAG
ATGCTCAATGTCCAGAACAAGAGCAGCAGCTACTTTGTTGAATGGATTCCCAACAATGTGAAGACCGCAGTTTGTGACATTCCACCAAGAGGCCTCAAAA
TGTCTGCAACCTTTATTGGTAACAGCACTGCTATTCAAGAGCTTTTCAAAAGAATCTCTGAGCAGTTCACTGCCATGTTCCGTCGCAAAGCTTTCTTGCA
CTGGTACACTGGTGAGGGCATGGATGAGATGGAGTTCACAGAAGCTGAGAGCAACATGAACGACTTGGTGTCCGAGTATCAGCAGTACCAAGATGCAACA
GCTGATGAGCAAGGCGAGTTTGAGGAAGAGGAAGATGAAGCTTGATGACAAAGGCTCCTAGCATTTTTAGAAATAAAAAGGCACAGTTTTTAAATAGCTA
GAGCATTTGCTCAGAATTTTGTTTCAGCATGCTTCTTCATTTTCTATTGTCAATGAAACCACAATCAGTTTTTTGTAACCTTTACAATGTCAAAGTAACA
GTTGTTTTTAGCATCTCTTCTAACGTTTACAAAATGCCCAAACATGATTAGGATGATGGTAGAAGTGATTTCGACCACTCATAGATGTTTTCCCCCACAC
TGGATAAGATCATAAAAGTATTTGTGCTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

5' EST: BQ736857 [+]

ggacgcgtgggttcttagccggctggaaaccatcggggtgttgcacaaagggcaagtaaagcaacaagaaagggccacaccatccactgattacaccact
gtaagactcaacccatcggaaacatgcgtgaaatcgtgcacctccaagctggccagtgcggtaaccaaattggagctaaattttgggaagtcatcagtga
tgaacacggcattgatcctaccggcagttaccatggagacagtgatttgcaactagaaaggattaacgtatactacaatgaagccacaggtaacaaattt
gtaccccgtgccatccttgtggatttggaaccaggcacaatggactctgtcagatctggaccattcgggcagattttcagaccagacaactttgtgtttg
gtcaaagtggtgctggcaataactgggccaaaggtcattacaccgaaggagctgagctggtggactctgttctagatgtggtgagaaaagaatctgagag
ctgtgactgcctacaaggttttcaactgaccncattctctgggtggtggcacaggctctggtatgggtaccctgctcatcagtaagataagggaagagta
cccagaccgaatcatgaatacattcagtgtgatgccatcacccaaagtctcagacactggtggttgaaccatataatgcaaccctctctgttcatcagtt
ggtggaaaatacagatgaaacctactgcatagacaatgaggccctctatgatatctgcttccgcacttttaagttaacaacacccacatatgggggatct
gaaatcaccttgtatcccgctaccatgaagcggggtaacaaccctgccttccgtttccccaggggcacccttaaagggttgatcttacccaaactgggct
ggtcaaaaatggggggcccttttccc

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Library:

Name: NICHD_XGC_Emb4

External Dbs: Unigene laevis cDNA library, Xenopus IMAGE cDNA Library

Description: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.1 kb. Constructed by Life Technologies. Note: this is a Xenopus Gene Collection library.

Anatomy: whole organism	Stage: NF stage 31 to NF stage 32
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pCMV-SPORT6 Type: phagemid 5' Restriction Site: NotI 3' Restriction Site: SalI

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Usage:

Whole mount in situ hybridization:	Expressed in Primary neurons.
Probes:
Type Size Promoter Linearization Site Description antisense T7 SalI EcoRI or SmaI can also be used.

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Suppliers:

Search for clone at: EXRC