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Summary Attributions
XB-CLONE-3233273

Clone Name: ptf1a-HAR413 Gene: ptf1a Species: Xenopus tropicalis  
Description: EXRC Clone Number HAR-413. IMAGE:7557710. Xenopus tropicalis pancreas specific transcription factor, 1a. NOT Full length. Accession number CX496418. To generate probe for in situ.

Sequence:

5' EST: CX496418 [+]

3' EST: CX496417 [+]

Library:

Name: NIH_XGC_tropGas7External Dbs: Unigene tropicalis cDNA library, Xenopus IMAGE cDNA Library

Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html). Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).

Anatomy: whole organismStage: NF stage 10.5 to NF stage 12.5
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pCS108

Type: plasmid

5' Restriction Site: SalI

3' Restriction Site: NotI		Expression Image

Usage:

Whole mount in situ hybridization:  
 
Probes:
Type Size Promoter Linearization Site Description
antisense SalI

Suppliers:

Search for clone at: EXRC