XB-CLONE-3390193
|
Clone Name: IMAGE:7651869
|
Gene:
wwc1
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
Full length sequence: BC136041 
[+]
gggatgcccctggattgctttggcacaggtgttcttcttttatacccctgtttagcaactggactttagcattcctgcttattgccccttaggtaaaaag
caccaattctgtttttatactccctgacggatttagaaccccctttgggcaaatttatgggggacagtgccctttctggagatgataaggaaggagctcc
ctctgccagaaggctgggaagaagcacgagatgtggatggcaaagtgtactacattgaccacaccagcaagactaccagctggatagatcctagagacag
gtttacaaagccattaacctttgccgactgtattggcgatgaattgccattgggttgggaagaatcttatgacacccaagtgggtgtgtattacattgac
cataacagccaaaccacacagatagaggacccaagggtgcaatggaggagagagcaggagcgcatgctgaaagactatctggtgctggcccaagaggctt
tactcgcacagaaagaaatttaccaagtcaaacagcagaggctcgaattagcacagaaagaatacaggaccctgaatgatgtctggaagcataaagtggg
atctcagacaagcttggtatctggctcctcctcaagttccaaatatgatcctgatattctaaaagctgaaattgcaacaacaaaatccagggtgaacaaa
ctaaaaagagaaatggtctttctgcgacaagaactccagtataaagaaaatggctaccaaactctcaaagagattgacttaaaaatgtctaaaaatcact
gcgcttataaacttgatgaagcccaagctatcctgaatgagatgaaaactatcaaaaaggcaattacttctggcgaacgagagaaacaagacctaattca
gagtcttgctgaattaaaaaatggattcctcgttgacaaaggatcacaatcagacctctgggccagcaatacttctttagcatcttcagctaattctctg
tccaggcagtatctcaatgtcagctctcaaacagatatttcaggaaatctttctgtggtcagtaataaccatttggccgagaaggtcaggctacaactga
gatatgaagaagcaaagagaaggatagcaaacataaagatccagttagctaaaatcgacagcgacgcatggccaggagtcctcgactctgaaagagaccg
gctcatactaatcagtgagaaggaagaactgctgaaagagatgcgctttatcagcccgcggaagtggagtcagagcgaggtggagcgcttagaaagtgag
aagaagcagctagaggaagacttacaggctgccagggacacacaaagcaaggcactcacagaaaggctgaaaatgagtagtaaaaggaaccaactggtca
aagagttagaagagacaaccaggttaattgcatcactgcaggcacaactaaagtgtctttcaaccagcatgcagtccctttcatcgggcagcagccctgg
atctttagcttccagcagaggttctttggtaacatccagtcaggactcctcaacatctgcgagtgtttctgatctttactacgagcaactggatcatttg
gattcagaataccagaacaaagtagacttcttaattcaggaaggagcaatgggttatagaccatctgggtgcatcacaaccattcatgaaaatgaggttg
tgaggtctcagaagactgatccagcaaatcgtgtgaaagcactgaggtctttatctggaacccctaagtcaatgacatcattatcaccgaggtcatctct
gtcctctccatctcctccttgttcaccgcttgtaactgatccactgttaacagtagatgcttttctgagtcaaatcgattttgaagatattgacctacca
ctgagctttactgaacttaatgtaaatgctggcggagatagctaccaacatgaaacagatgaagaaccttcaggacaaggtttaagctgcttgggggtgt
ccagtctgagtgtggccggagtgtcggctgcagtgtcagatgaatctgtaactggggacagtggagtctacgaggcaacgaatcagagaacattgatgga
aaatgcagtgtttgatggtgaagactcagagatgatggacaccgccaagatcaaaatagggatgaagtatgatgagaaaaacaagcactttgctgtattt
gtcattcagttggataatttggcagctttgttactgcatcagaatgaaaaactgtacatccgcgttgcagttctcccgtgttctgaaagcacgagttgtt
tgttccggacaagaaccttggagcagtctgacactcttctctataatgagatgtttctggtttctacatcccattctgctttacgccagaagaccttaag
ggttgatgtgtgcacaagacaaagcaatcatcatgaagaatgcctgggaggatcacaaataagcttggccgatgtctgccattcagaagaacaagaagcc
cattggtacaaccttttaagttaccgatatctgaagaggcaatccaaaacaagcaagcagaaaatcagttgctgtgaagaaagtggtattgaaaatccag
attcagtgagtgccctccttaaacagactgctgctgaattagaggctgtaaggaagcagattggggaaagcacagatccacctgaatatatctggccaga
aaagaaggataatgtcccggatgaagatggcattagtgagaatgaaggagaggaagaatttgatggagatgtacagtgtgaaacagaagaatcagacatg
caggcagctacagattcagcctcggtaaaggtggacaaggagacaaacacagaaggccttgcaatgtccttatcttctgttcgcccaaaagataaaagag
cagccactcctatgcaggcgccattcataagaggaaacaccattatacgctctaaaacattctctccaggaccacaaagccagtatatttgtcgtctgaa
tcgtagtgatagtgaaagttcaactctatctaaaaagccaccttttgtcaggaacgcaatggagagacgcagcgtgagagtcaaacggccatctgtgaag
tccactggctcagagcgtctcattcgcacatctctggaccttgaattagatctacaagcttcaaggacatggcaccatcagctcactcaagagatctctg
tacttagggaactaaaagaacagttagagcaagcaaagcgcctgggtgagaatgagctacctcagcgggtgaaggatgatgagacatttaaggttatgct
cagacaagtggagaaacaggtacagaagattgagcagaagtacgagatgcaagcagataagatgatgagagcagcagcaaaagatgtgcacaggttacga
gggcagagcagcaaggagccattggaggtgcagtcattcagagagaagatggcatttttcactcgaccaagaatgaacttacctacactcacagcagatg
atgtttaataatcaccataaagtattttttgtttttctgttctgagcgccactgcaatgcaaagtgcagcccggtgcctcggaaatgtcattgtgtggtg
taatgaaggtacagagtttcatgtccttttttattttgggttgcaataaccagcacagtaacactggcttcaggtaccacaacatcagcgaacattttgt
ttttttagtcattattacaaaaccagcattccttttccaactttgtatagcgtgtatatttagggctgtattatcagataaactgtttgaaagagaatca
gatgttgttgtgtttgcaaagtatcattttgtacagtccgctgcagaggtttaaaatgcaataaaattaaattaaaagagacttctactaaaaaaaaaaa
aaa
5' EST: CX814487 [+]ggatgcccctggattgctttggcacaggtgttcttcttttatacccctgtttagcaactggactttagcattcctgcttattgccccttaggtaaaaagc
accaattctgtttttatactccctgacggatttagaaccccctttgggcaaatttatgggggacagtgccctttctggagatgataaggaaggagctccc
tctgccagaaggctgggaagaagcacgagatgtggatggcaaagtgtactacattgaccacaccagcaagactaccagctggatagatcctagagacagg
tttacaaagccattaacctttgccgactgtattggcgatgaattgccattgggttgggaagaatcttatgacacccaagtgggtgtgtattacattgacc
ataacagccaaaccacacagatagaggacccaagggtgcaatggaggagagagcaggagcgcatgctgaaagactatctggtgctggcccaagaggcttt
actcgcacagaaagaaatttaccaagtcaaacagcagaggctcgaattagcacagaaagaatacaggaccctgaatgatgtctggaagcataaagtggga
tctcagacaagcttggtatctggctcctcctcaagttccaaatatgatcctgatattctaaaagctgaaattgcaacaacaaaatccagggtgaacaaac
taaaaag
3' EST: CX814486 [+]tttttttttttttttagtagaagtctcttttaatttaattttattgcattttaaacctctgcagcggactgtacaaaatgatactttgcaaacacaacaa
catctgattctctttcaaacagtttatctgataatacagccctaaatatacacgctatacaaagttggaaaaggaatgctggttttgtaataatgactaa
aaaaacaaaatgttcgctgatgttgtggtacctgaagccagtgttactgtgctggttattgcaacccaaaataaaaaaggacatgaaactctgtaccttc
attacaccacacaatgacatttccgaggcaccgggctgcactttgcattgcagtggcgctcagaacagaaaaacaaaaaatactttatggtgattattaa
acatcatctgctgtgagtgtaggtaagttcattcttggtcgagtgaaaaatgccatcttctctctgaatgactgcacctccaatggctccttgctgctct
gccctcgtaacctgtgcacatcttttgctgctgctctcatcatcttatctgcttgcatctcgtacttctgctcaatcttctgtacctgtttctccacttg
tctgagcataaccttaaatgtctcatcatccttcacccgctgaggtagctcattctcacccaggcgctttgcttgctctaactgttcttttagttcccta
agtacagagatctcttgagtgagctgatggtgccatgtccttgaagcttgtagatctaattcaaggt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
