XB-CLONE-3915169
Clone Name: IMAGE:7091860
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Gene:
slc5a2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653856 [+]
taattaatcccccccccccccccataacagaaggaacacaagacttgagggtagaaaagaagtggaagctgaagagagacagtgccaaccagcagacaaa
ttagagagccatggagagcaccattataccagagacaacttcgcatatctccccgaagggcaacgtggccatcagcaacccagcagatatctccgtcatt
gtgggatactttatcattatcatcggagtgggcctgtggtctaggcgccgagccaacagggggacggttggtgggtattttctggctggaagaagcatgt
cttggggtccggtcggcgcctcactatttgccagtaacattgggagtgggcattttgtaggactggcgggtactggggcagcaagtggcattgcggtggc
tggatttgagtggaatgctctgttcgtggttctgtttctgggttggatatttgtcccagtctacctgacagcagaggtggttaccatgcccgagtacttg
aagaagcgctttggggggcagaggatccagatgtacctgtcggcgctctccctggttatgtacatattcaccaagatctctgttgatatgttctctgggg
cagttttcatccaagttgccctgggctggaatatttatttgtctgtgattgccttgttggttaa
3' EST: CN329368 [+]ttttttttttttttaaataacacgcaatttattaaatgcaattaatatattacaaaccctcccctgcactgtgtcatgcaaaatatgcccacaagaagat
ggcgcaggacatcataatcaaggcgttgatattcaccaggcgggcatacttggggtcctctcggatatctggaaggtcggacaactgcttctcctgggtc
tgaggatcgctgacccctgtctcctccatttcttcccgcagctcctggctgtgccggaaactatagaccaagcgatggagcttcttctctgggattggag
gggtgcacagggatacgatcaggacgatggctccagaacacaggaagaaaatgatggcaaagtacagatagtgaaccccacagattatggttgggcagga
gcttggggccgaacagcttccaaatccgaatataaactcgggcaccatacggcacaagcccaggaacaggccgccgaccagtccccagaatgcacccggc
tcattgactctcttcacaaacagcgcaaggaaatagacagccgcaatgggcggaaccaggaaacttgccaccgactgaatataatcaaagagctgccctc
cttgtgcagcctgaaccacagggatccacgctatactcagtgccacgaggcagactgtccacaccccttcccaccacagcagctccttctccctcgcccc
ggggcccccatccccctccaaaagtccctaatggaaaagagacccactggcttttaaaattggaatcccaggctcccccttaaaaacggcccaaattccc
ggcccaccaggaatcccccgagaaacctttttggcttgaaattcttagaaccccctttttgggga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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