XB-CLONE-3915841
Clone Name: IMAGE:7091894
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Gene:
srsf2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654294 [+]
taattaatcccccccccccccttcgccttcgttcgcttccacgacaagcgcgatgccgaagacgcgatggacgccatggatggggccgtgctggacggcc
gggagctgagggtgcagatggcccggtacggccggcctcccgattcccaccacggaaggagagggccccctcctcgcagatatggcgactacggccggcg
gagcagaagccccaggaggcgacgtcgcagccgttccagaagcaagagcaggtcaaggtcccgcagcaggtctcgctacagccgctcaaagtcccgctct
cgcacaaggtcccgcacccggtccagctcaaagtctcgctcggcaaggagatccaagtctaagtcatcgtccgcgtccaggtctcgctcccgctccagat
ctaggtccagaacaagaaattcgccgccgcctccacagaactccaactccaaatccagatcccgctcccagagccctcctaagtcgccagaggaagaggg
cgccgtgtcttcctgaagagtcaacagccggaattctacaagctggacaacatccctggggtgataattcattcattagctcatcagcccacggagaaac
acagaacaaagctatggcctgccgaaaaggtggttatttgtatgatttcttaaactttttaccggttttcatttgccctcgtgtcgcagaattttacttt
ttagggcaatttgttcctggtatcccatttctgtatttagga
3' EST: CN325180 [+]tttttttttttttttatttttttttttttttttttttttttttttaaagaaaaaaaaaagtttatttactacagaaacagtggttatagtagagaatgag
agccagagatatgcagatcagtacatacaaaggaatgacaccagcatgggatattatcacacaatgatgctcaggatttaccaggtttaaaacataaagg
ggtttctcatcagctagatgtgctcactgtatgctccgttattatatgcaaggcccgggcgactggatgtgcagttgtcgtcaggcatcctaataaactg
gacagccattgggttttctgcacgacaaggcagcacaggagcaatcaggagataacaggaaacagccaagcactctgcactgcaacacgccaccttaaac
agctaaccagcaatacaactgctacacaactgcgcctagtgcacaaaaatacacaagagaagaaattagagttgtaatcaaacaaaaacactcccttgca
acattgtcacatgaacattttttacccccacacttcaagtctcacgtattacaagaacatatgactgggtggaaccctggccgggcaatctgacgaggca
cagcgcccatgcactataagtagggctgtggagcatactagctgtgagataaccttcattcccccacagaatttcaatgatactttgacaaaagcaagtt
acctcaaacatccagcccatattgaaatctgtagtcggtccttttcactaaattacaagaaaatcggaaaacaggggaacaaaatggcccataaaaggaa
taaagtcctggcgaccccaaaccccactggaaaaaaagggtaaaaag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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