XB-CLONE-3916347
Clone Name: IMAGE:7091962
|
Gene:
ptma
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CK654565 [+]
taattaatcccccccccccccgactggcagtcattataagaaataaaaagccatctttgcattgttcctgtaacactcgcttccagctcccgggacctgc
acaattactttggttttttgtatatagcttattttattatattaagaatgtccgacacagcagtggacgccagcgttgagaaaacccccaaggacttaaa
agcaaaagagaaggaagttgtagaagaagcagaaaatggaaaagacaagcctaccaatggaaatgcagaaaatgaggaaaatggggagcaagagaaggaa
ggtgatgaataataagaggtagatgaggaagatgaagaggatgaagtagaaggagatgatgatgatgaagacgacgaagtatagggagccactggcaaga
gagcagcagaagatgatgaggatgatgatgatgatgttgaaacaaagaagcagaacacagatgaagacgactagagtggggaattgtccctgtctcanaa
taaagtctatatataaccttacccccaccgcctgccctcaactctcaaatatgggtcacagaaagtagttcttccgtcccgacccctcatgcaacctctt
tggcccccatcctcctccacaaatgacaccactccaatgtgccagtccaatggccacaaggtttgtttttgttaaattttta
3' EST: CN322119 [+]tttttttttttttttcttttttagaacaaaaggaaagcctgcaaattaaaattgcagttttattgacaatactgccacacacatccaatcaggccaaaca
aaacaaaacaaaaaaaaagcaatttcagagacaaaaacaaaagaaaaatctttggccattctcatccaaactcatctaaagtacagctaaaaatatgttc
aaaaagttactggaatgatcagaattttatattgggggttttgtcttatttattttaagttttttttataacaatttttgctttttttgtaaagattttg
ccatttggagaagagacagctctcacctccacacctcccaccgcccttccccccaacccagaaaagggggttaaaaaatgcaaggttttttttcctccat
atatatagccatgggaacaattatcaaaaaggagggggggggggggacaaaaaaaaatattaatttaaaagcccccccaacaccctggcagtcaaatccc
cagtacagaaaaggacagttgtataccaatatgtacaaaaatataaaatgtaaataaaaaatacaaacagaggaccccttccaaggcgtttgttttccca
gttttttataaaaaggaaaaaacaaaataacccctctagaattttaatggaacaattcaccccccccaaacaacattatgacgggaaaggaagcaaaaga
gtgctttctaactgcagggcaggccaaacccgggcgaaatgacccaagggtgttggaaaaaaaaaaaattaaacaaaaaaaaacttgggggccctgggag
gggccctttggagggggggcttttggggaggggggagggggggcaaaaaaggccgctggaggggcggggagaaaaaattttttttttggatccattttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: