XB-CLONE-3916691
Clone Name: IMAGE:7092055
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Gene:
ptmap12
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653822 [+]
taattaatccccccccccgattcctctccactgagtcgtgcggctgctcggtgactttgttccatccattcgtcacattttccccgctccgaaaccaaag
aggtctttattttttcctcttttcccgaattaagaaactctcaaaggaaaatggcagataccgacgtagataccaccaccgccgaggtccccgtcaccaa
ggatcttaaagaaaagaaagaagtggtagaggaaccagagaaggcagagaatggcaagggagatgccccatctaatggcacagaagagaatggcgctgat
catggtgcacagaatgatgatgcagaggaagaggatgaagaaggggatgtagaaggtgagggtgagggtgaaggagaggaagaagaggatgaggaagaag
gtgaaggagatgaaaatgagacagatggtctttcagtaaaacgtcctgcggaagatgaggagaaaactgaaactaaaaaacagaagacagaaaatggaga
ctccacagaagtgaaagaatctgcctaaattctccttccagacattttatagcttaagtgacatggttgactttaaattgtagagaatgtgctttatatc
tgtacattttacaaaatgactttttatatactgtaaacttacatgcactagaaacctgtaacatcacagcaagatagctaaggttttttgtaaatgtatt
tattggggctggttgtatgggtaact
3' EST: CN320061 [+]tttttttttttttttcttttttttttttttttttttatataaaagatttatttatagtacgtaacctgaatatacaaagtagtggtaagcataaactaat
gaggtatacagtttttatcttggttataactgatgaaaactacactgtcaatgcctcaaaatttctgcagaacacacacactttgatagcacattaggat
acagagaaatttacatattgaaccttgcaattgtcaagccttgcttgcaccacaactatataccaaacagcaacaaaattaagcccaaagatctatacag
aattcccaggttaaccctggctaaataagatccacatctttggccttcattgcatatgaactttctagcctaccaccattatattacaggaaggcagctt
cactcaagaatacacatggctgtactaatatgataagacatgtgcttcaatggtgtacaaaagcaaagctgtaccacttaaacaaaaaaaaaaaaaactt
tagatttttattgaagcagttaaccagtacaaacaagcaccaataaataacattttacaaaaaaccttagctatcttgctgtgatgttacaggtttctag
tgcatgtaagtttacagtatataaaaagtcattttgtaaaatgtacagatataaagcacattctctacaattttaagtcaaccatgtcactttaagctat
aaaatgtcctggaaggagaatttaggcagattctttcaccttctgtggagtcttcattttttctgccttctgtttttttgttttccgtttttctccctcc
tcctcccgcagggaagttttttactgaaaagaacaatctgggtcccattttttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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