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Summary Attributions
XB-CLONE-4272575

Clone Name: id3-HAR54 Gene: id3 Species: Xenopus tropicalis  
Description: EXRC Clone Number HAR-54. TGas044c15. Xenopus tropicalis inhibitor of DNA binding 3, dominant negative helix-loop-helix protein. To generate probe for in situ and mRNA for injection.

Sequence:

Full length sequence: CR761665 [+]

5' EST: AL655021 [+]

Library:

Name: XGC-gastrulaExternal Dbs: Unigene tropicalis cDNA library

Description: cDNA was oligo dT primed from 5ug of poly A+ RNA from stages 10-13 gastrulae. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.

Anatomy: whole organismStage: NF stage 10.5 to NF stage 12
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pCS107

Type: plasmid

5' Restriction Site: EcoRI

3' Restriction Site: NotI
Expression Image

Usage:

RNA injection:  
 
Probes:
Type Size Promoter Linearization Site Description
sense SP6 AscI

Whole mount in situ hybridization:  
 
Probes:
Type Size Promoter Linearization Site Description
antisense T7 EcoRI


Suppliers:

Search for clone at: EXRC