XB-CLONE-4350118
Clone Name: IMAGE:7025772
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Gene:
phyhdlb
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785727 [+]
taattattccccccccccccccccgagacttacatcccagctacatctcagcattactgtacagcagaaaatcctgtccctgagccatgaaggcagaagc
cactcagctgaaggctctttatgaagctaacggcttcctgacagctattgatgtgctggggaaggactagctcgatcaggcctgcaaggaacatgcaaac
ccggagaacaaattcggccgggaatacacacagtacaacctgcataacattcacatgcaatacgagtgggtgatgaatcaggctgctcatccaaaacaac
cggacgcaaacactgcagtgatcagggccaaatgttatcccccccgccccctggttttatctgaagtacattttctttgacgtcctaattacacgccacc
cccctgtcctatacgggtgtttggcattcgcgacttctaagtaaaaacgcgggctttaaaggagaggcttcccccgcaagccccgccccgcatatgggat
ttttaataggaacgttggtcattaccgagaaacaccgggcatttcactgaaccacgttttatactccgattaagccggccgctacagttatttccggggg
ggaatcaatttttgtgaaaaatacaccacaccaacggtgcgctctgttgaaanaatattgccctaaagagagaaatgaattatgtattattctttttttt
acgtatgtgatacagatattcacccgcacgcgcagtcagattaaataattaaaatcccacacacgggatgggggagattcgttttttttgtgtaggttcg
cgcccaaggggggagttgagctggaagagtgagttaatttttaagcgaccgctatcgcgaggtcagcgaccgtatacactatcatttttgtcctagggaa
gatgcctgccgtgtggaaagtctatcttatattctgattgtccgcatgcgcagcttcatgtgcatatattcgggtgtcaatagaagacgccccacttatt
aattactaattcgacacgtctagtacaccgcctctttttatatgacagtgttgcttcgccgttatatatgcttaccacggcaggtccgactatttttttt
tgggtggtaccagggcggttactcgccctgctttttaaacgacggcggagcgcgtgttgtttttcnatgtagtttaaatctggcgtcgtctcctgtcgta
tctgtatttccaatcaatatgcgcaggcccttcgatttcttgactaatattttaactcccccgccgcccaggcgttg
3' EST: CF785728 [+]tttttttttttttttgccaagatgtggaatttattgagcagaacagaaaaaaacagtaataatttattatcttttcaaaataactgcataaaaacactta
atacttttaaataacaaataaaatacatttaaaataatgactggcaggactgtagcaagagacttctctgcagatacggaccagagggaagaggtggata
tatgggggcctgcccacagctgcagaattcccaataaacccctaggaggctagcatagggcaaggccccagaatttctggttgtacctgctccagcaagg
aaaaatgccaggccagtgtatccccactgcatggggtgtccccacattttaaaaacatgaactatgaggtaaatgtgaatttcagctaagcatccaatac
agtacatcaatgtgagcattaaacagatcactgcacatattcactgccataatgtaaatcagtgcctcttgtttatacagtacagagtgggcaaaaaaaa
ttcttctccttttgagttgtaaaaacacaaaagtatgagacaactgaaggcagttaataggtcttgttaaaaaagttaggagcgtggtcttcaaagttct
taaactcatcagttccagcccatatgtagagaan
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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