XB-CLONE-4350421
Clone Name: IMAGE:7025646
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Gene:
epb41
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785536 [+]
taattaatccccccccccccccccgggtagcagacccgaaatacggtaacaatgacaacagagaagggattattggctgaggcagaaagtcctcctcagg
accaaaagcaagaagcagaggaggaggtagaaggttgtacaactcaaccagttggtagcagttgtgataaggatccagagaccgaacagtcacaagattc
accttccaccacctctccaagcacgcgcaagatcaaggatcgacattcagaaggaaagggcctctctcgccttttttcctctttcctgaagaaacccaag
tcccaggtgtcttctgatgaaaaggaggttgaactgttggaggaaaagggacaagaccagaaggatgttgatgagggtttgggagagcagctagaggatg
atgtttttctgaaggcccctattgcagctccagagcctgaactacgaactgacccttcactagaccttcattccttaagtagtgcagaaacccagccagc
tcaagaggaacaaaaagaagaccaagacccagaagcagattgtgaggacgtggaaggaaaagagcagataaaaaaacctggaaggagagtcaaaggcctc
accagaaagtaattaggcgtttctacaaaatgcactggaaagtcacactttctcgaataacactgtttatgaaatgtggacttggaagaaacattgcaaa
ggggtcagggacttattttaaaaaaagtatggcggcccttctataattattcgggaaggaaagattaatttttggaattgggcaatgggggggaaacctc
3' EST: CF785537 [+]agagacagaaacctatgcttgggaatagactctgtggatgtcaacctaaaaaacgtatgatgttccacacatactttccaaagtttctttgctgccttat
agctgggaagcttaaatcctattgtactttcatattgctcctgctctcctgggcgaattttgatgaagaaactacttcttttatatgagatcttcagtac
tttgggccacggaaacctgttaatcctcagattgtctttgaagaccataagaccaccagagcaaacaccaagtttgatatccactccctctaagtccttg
gcttggtgaatatctacaccatacatggtaagcttttttgcattctccaggaattccaggtcagcctgagcaggtgtcatagatctgtatgacttgtgaa
gctctacaaccttctcctcaagatctttagtctgattcggactgagtttaaattcgttaacatagtctacaccatgaagatcctcttcatagtcacccac
ttctgactgaacagtataagaaccaagcagagcaagagtggcaaatgagcagggaagacacccactgaaaatatctttgcgtagctggagacacaggtag
tacctggtaatgtcctctgacaattgtgcaggatctggnggataaaacttcacatttgaagtgaattcacatggacctccatgaacctgcttcctcatgt
ccttcaaaggatcaagccagaccttgcaagttggagattcccacatgccaatccaaaataatctttcttccanaatattaagatggctgcatactttttt
taaataaagtcctgaaccctttgc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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