XB-CLONE-4350449
Clone Name: IMAGE:7025657
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Gene:
dynll1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC076999 [+]
GAGTGTGGGAGAACGCGGGTTGTTACAGTTGGTGCGTTGCTGTAGAAGAAAAAAGTCACACAGATCACCATGTCTGAGAGAAAAGCTGTGATCAAGAATG
CTGATATGTCAGAAGAGATGCAGCAGGATGCTGTAGATTGTGCTACTCAGGCACTTGAGAAATTCAACATTGAGAAGGATATAGCAGCCTTTATCAAAAA
GGAATTTGACAAGAAATACAACCCTACATGGCATTGCATTGTGGGAAGGAATTTTGGCAGCTACGTTACACACGAAACAAAGCATTTCATTTATTTCTAC
CTGGGTCAGGTAGCCATCCTTCTCTTCAAATCTGGCTAGACTGATGAGGAATCCTCACTTTGCCTGTAAAAACTCCAGAGACTTGTGCTATAGCCTAACC
AAGGGAACAAACCTTCTCTACCTTCCTTGTGTCCTTATTTGTGATAACAGGACTGAAAACCTGTTGTGCTGAGTTGTTTTTCATAATGGTTATAAAAGAG
AATGCAACAACAAAAATCCGTATTTATTTTCTAATGTTGCTATTTTTTTCTTAACATTTAATAAATGTTTTTGTTTTCTTTTGAAAAAAAAAAAAAAAAA
5' EST: CF785519 [+]taattaatcccccccccccccgagtgtgggagaacgcgggttgttacagttggtgcgttgctgtagaagaaaaaagtcacacagatcaccatgtctgaga
gaaaagctgtgatcaagaatgctgatatgtcagaagagatgcagcaggatgctgtagattgtgctactcaggcacttgagaaattcaacattgagaagga
tatagcagcctttatcaaaaaggaatttgacaagaaatacaaccctacatggcattgcattgtgggaaggaattttggcagctacgttacacacgaaaca
aagcatttcatttatttctacctgggtcaggtagccatccttctcttcaaatctggctagactgatgaggaatcctcactttgcctgtaaaaactccaga
gacttgtgctatagcctaaccaaggaacaaaccttctctaccttccttgtgtccttatttgtgataacaggactgaaaacctgttgtgctgagttggttt
tcataatgggttataaaaaaagaaggcaacaacaaaaaacccggaatttatttttccaaatgttggctaattttttttcttaaacattttaaaaaaaggg
tttttggtttttccttttgggaaaaaaaaaaaaaaaaaaaagggggtcccacctaaattttctagaaggcggggccggcccccccccccgggtgggaaac
ttttggggccccggggggcccacaaattttggttttatatgggcccggccttatataaagggggtttcacaaaagaagaagggccatatt
3' EST: CF785518 [+]ttttttttttttttttcaaaagaaaacaaaaacatttattaaatgttaagaaaaaaatagcaacattagaaaataaatacggatttttgttgttgcattc
tcttttataaccattatgaaaaacaactcagcacaacaggttttcagtcctgttatcacaaataaggacacaaggaaggtagagaaggtttgttcccttg
gttaggctatagcacaagtctctggagtttttacaggcaaagtgaggattcctcatcagtctagccagatttgaagagaaggatggctacctgacccagg
tagaaataaatgaaatgctttgtttcgtgtgtaacgtagctgccaaaattccttcccacaatgcaatgccatgtaggggttgtattttcttgtcaaattc
cctttttgaataaaggctgccaatatccctttcctcaatggttgaaattttctccaagggccctgaattacccccaatcctaaaaggcatcccgtgccgg
caatcctcctttcggaacatatcccgccattcccttggaatcacacggccttttttccccccccaaaaaaaagggggggaaatccgggggggggaaactt
tttttttccccttcccaaaaagggcaaaacccccccccccaaagtgggataaaaaaaaaccccgggggggtttttcccccccaaaaattttttggcn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: