XB-CLONE-4350537
Clone Name: IMAGE:7025696
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Gene:
XB5729910
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785462 [+]
taattaatcccccccccccccgacttttgacagtcttgtgatcacagctgcacttcccgggagagaatgaaacttcattcaacgttttagattttttaat
tctggctgctgtttcatacatcatgactaccaccttgcgttatttgtgctgctgctgtgcatctggcagtgaggaagagagcaccccttttgcacgagaa
ggaagaagccaaaaggcaatcaagactcttaacaaaggtgaaaaattagttatgaagcatgtcaacgtgccaagcattgataaccagtttgcagagatcg
gtaatctctacaatgatcaggtggagcaccatgagaccatgacacagtgtatctctagtctggcacatatcagtcattcatctccagacctcaccgcctg
tatacagaggctgaaagacaactattatgactatgatattaaagtactgatggaagggtacaatttctctcttctggtgaaaacagaaaaggaacctcca
gaaaacttgaaacaagcacaggagatcgttaaaaagttaagtcgtgcatccaaacttctcatcagtaaccaaaccagactggggagagatgatgttcaca
gttctgcagtcacaaaataagactgatatggaaagcagcataaaggaggccaacactgtttacatggaccatttacgcctaaaaaaaaacttggaagaca
aatctacaaagacagacnaagcccgacaactgtctaaagaatatgaaggaggatgcccacaaaattcttatagaattatcctagaattccgggagctgca
cctttgaaaatatca
3' EST: CF785463 [+]tttttttttttttttctttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttt
ttttttttccccctttttttttttttttttttttttgaaaaaaaaccccttgggggggggggcaaaacccccaaaaaaaaaaaaaagggggaaaattggg
ggccccccgggggacccccaaaaaaacccccccaaaaggggggggcccccccccccccaaaaaaccccccccgggggggggaaaaaaaaaaacccaaagg
gggggggggggggggggcctttaagggccaccccccccccccgggaaaaggggggggttttttttagggggcccccccccaaacaaaaaaaaaccccaaa
aaaggggggggggcccccccccccaaatcaaaaaaaaaggggggggggtcccccccccctaaaaaaaccccccccccctttttttttgggggaaagggcc
ccccaaaaaagggggggcccccccctggggggccccccaaaaaaatttttttgggggggggggaagaggggaaacccccccccccccccggggggggggc
ccgaaaaaaaagtgggggcccccccccccttttttaaaacccccccccggggggggcaaaatgcgggggggggtccccttggttttcccccaaaaaaaaa
aaaaaggccccccccccccccgggtttttattcacggacgcccaaggggggcctccccccccccccccaaaaagagggggggggggcgcgcggaaaaaaa
aaacaagattttggcccccccacaaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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