XB-CLONE-4350552
Clone Name: IMAGE:7025701
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Gene:
atp6v1c2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785453 [+]
taattaatcccccccccccccaccccccngggaaaagcaggacatacaggtgagagctgccactagctgaggactgggagcagttgaacagagaaagagg
aaaccataaggactggaggtagaagaaagataaggataaagaaagggacaggtgcatatcaagtacttcagagaagcggaatcgtcgtttttttcctcca
gatccactggtggagtcaggttttgattcaacctacagagggtgcttgctatgaaaagaacatttctacgccatcaggaaatcaagtaaatattttgatt
gaaaatgtctgaattgtggttaatttcggtgcctggtgataaaaccaacttaacagctttggatagaaatgaatacagtgacttccaaagctaacctgtc
cagcaatgccaggtttgtcataccaaaactaaaggtggggaatttggatgccccttgttggactttcaaaggaattggggaaaaatcgattgcgtattgc
tgaaaagcttggaataaaaagaaatcgcccatttacttaaggggaaagatcttgggagtgaatttaacatggcaaaaaattcctatggaaaaattttagc
cgggggcaatgggagtggggaacattatcagggctacactgacaccccccatctgcgaggggggcaatggggctaaagtatacctggatccagggttggc
cccgctgggacgaacatgtaaagtgaaaggttttaggggacacacgctacccggtctccccgatagtaaagacgaatggttaaacgggttcgcggcacaa
ctcagggctttttccaaaaggactagtttatgattggagaccccctcggttcagaact
3' EST: CF785452 [+]gccaatgaaagcttcactgaaatttactttcagccagcgcagcaggggaccataaagctgttttttatcagccgccagtttaacgatttcagctttttct
gactgcagttccttctcgttaaagagaaattcccgaacgatgaatttgttttcccgggccttcgctttaaagtcatccattacttttctaaataaggtca
ctgtgaaaaggcctccttctgcatcctcggcaatcattttggtcgagcgtggaaccaccatatcagacagcgattcatacgttttttgccatgcaccata
acttgactttggaacaaccacaagtagtgtgaccagatattctgagtctaggacaaagtcttctttattcactatatcagctaaggttcttgtcaagagg
ttgcccacagttttcctctccaggctttgcaggctccctttaatgttattgtaagcagaagacctggactttaaatctgtgtctatctgtgacatttgct
tgcttaatacttccattatgttcttcagtggttgcttgactggatacttagccatgtcccactcaaagcgagctaagtagctgataaggtccactccatt
tgccagcaaattttcttgggctttgtcagttgaatcatccatgacttctcctatgtattgggcaatcttcttaatcaagctttcagcatacgcatcgagt
ttcccccattcatctgaaagtccaacaagggcatccaaagtccccaccttttagttctggtatgacaaactttggcattgctggnacaggttagctttgg
gaagtcactg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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