XB-CLONE-4350652
Clone Name: IMAGE:7025937
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Gene:
rpl7a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785391 [+]
taattaatccccccccccccgcttttctctgcccctgtccaccatgcctaaagggaagaaggccaaggggaagaaggtggcacccgccccttccgtcgtg
aagaaggccgaggccaagaaggtggtcaaccctctgttcgagaagaggccaaagaacttcggcattggtcaagatatccagcccaagcgtgatctgaccc
gctttgtcaaatggccccgttacatccgactccagcgccagaaggccatcctgtacaagaggctcaaggtcccacctgcaatcaaccagttcacccaagc
cttggaccgtcagacagccacacagctcttcaaactggcccacaagtacagacctgagactaaacaggaaaagaagaaaaggctgctggccagggctgag
cagaaggccgctggcaagggagacgttcccaccaagagaccacctgtactcagagctggtgtcaacactgtcaccacccttgtggaaaacaagaaggctc
agcttgttgtgatcgcccatgatgtcgatcccatcgagctcgtcgtgttcctccctgctttgtgccgcaagatgggagtcccatactgcattgtcaaggg
caaggccagactgggccgcctcgtgcacaggaagacctgcaccagcgtagcactcacacaggttaacccctgagacaaaggtgccctcgccaagttggtg
ggaagcagttaagaaccaactacaatggaccgattcgaatgagatcccgtccgtcactgggggggtggtgggtatcctgggggccccc
3' EST: CF785390 [+]ttttttttttttttctggagtttatttttttttatttctgtacagaacaaaacaacaagatcggtacatttaaccaagcttggtggccagctcctttgct
tttgccttctccagtttggcgatgcgggcaacagacttggggcccaggataccaccaccccagtgacgacggatctcatcgaatcggtcattgtagttgg
tcttaactgcttccaccaacttggcgagggcacctttgtcctcagggttaacctgtgtgagtgctacgctggtgcaggtcttcctgtgcacgaggcggcc
cagtctggccttgcccttgacaatgcagtatgggactcccatcttgcggcacaaagcagggaggaacacgacgagctcgatgggatcgacatcatgggcg
atcacaacaagctgagccttcttgttttccacaagggtggtgacagtgttgacaccagctctgagtacaggtggtctcttggtgggaacgtctcccttgc
cagcggccttctgctcagccctggccagcagccttttcttcttttcctgtttagtctcaggtctgtacttgtgggccagtttgaagagctgtgtggctgt
ctgacggtccaaggcttgggtgaactggttgattgcaggtggggaccttgagcctcttgtacaggatggccttctggcgctggagtcggatgtaacgggg
ccattttgacaaagcggggtcagatcacgcttggggctggatatcttgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: