XB-CLONE-4351393
Clone Name: IMAGE:7026992
|
Gene:
mt-co1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784921 [+]
taattaatcccccccccccccctgtggctattactcgttgattattctcaacaaatcacaaagacatcggcaccctttatttagtcttcggtgcttgagc
aggaatagtaggaacagctttaagcctccttattcgagcagagctgagtcaacctggaaccctattaggggacgatcagatttataatgtaattgtaact
gcacacgcctttattatgattttcttcatggtaatgcctattatgatcggcgggtttggcaactgactcattccattaataatcggggcccccgacatgg
catttcctcgaataaataatataagcttctgactcctacctccatcattcctattactcctagcctcttcaggagtagaagctggagccggaacgggctg
aacagtctatcctccattagcggggaatttggcacatgccggagcctcagtagatttaacaattttctccctccatttagcaggggtttcttcaattttg
ggagctattaattttattactacaactatcaacataaaaccccctgctatatctcaataccaaacgcccctgtttgtttgatccgtactcattacagctg
tccttcttcttctttccctccctgttctagcagccggaattacaatactactcacagaccgaaattttaaaacaacctttctttggacccctgctggggg
gaagttgaccccattatttataaccaacaccctatttctgaattcttttgggcccccccagaaaattctaaaattttttaaattcccttcccaggagatt
tgggggaatgaattcctcccccaacatttggtggaaccctaaattaactttcaggg
3' EST: CF784920 [+]tttttttttttttttaggtgttgatttaaaagaaaatggcagagtggtgatgcgactgacttgaaatcagattatgggggttcgattccttcttttctcg
gattaattatgtgatggatgaatttgtacaaaggctggttcttcgaatgtgtggtaagggggagggcatccgtgaagtcattcaatatttgttgaagtta
gttctgttagagtgacttcccgttttgctgcgaatgcttctcagataatgaatatcatcataattaccgccactagggaaattaaggagccgacagatga
gacggtgtttcaaagtgtgtaggcatctgggtagtcagaataacgtcgaggtatacccgctaatcctaggaagtgttggggaaagaaggttaggttaacc
cctgcaaatataacgccgaagtggatttttgctcatgtttcgtgaagggtgtaccctgtgaataacgggaatcaatgaacaaagcctcctataattgcga
atactgctcctattgagagaacataatggaagtgtgctacgacataataggtatcgtgaaggacgatgtctaaagaagagttggcaaggacgatccctgt
tagtccgcctacagtaaatagggaaaataaaagcaagggctcaaagcatttggggcgtctcatttgattgttcctccatgcattgtagctagtcagctaa
atactttaacccctgtttggaattgcgaataattatgttgcctgaagttgaagtaggcttcgggtatcaacaatttaaatctacc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: