XB-CLONE-4351399
Clone Name: IMAGE:7026994
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Gene:
dll1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784917 [+]
taattaatcccccccccccccggcagtgtagtttgggacaagccgctttgcgccttgtgccttgtagcgctatcgcaccggcaagtgctgcgaggcatat
cagccaacatgggacagcaatgcatgctgactctcctcgtcctcttcgccgtactctgtcagatatcctgctccggacttttcgaactcaggctgcagga
gtttttaaataagaaggggctgctcggaaatatgaactgctgcagacctggatcgcttggcactttgcagcggtgcgattgcaagaccttcttcagaatc
tgccttaaacactatcaaagcaacgtgtctccggagcctccatgtacatacggcagcgcggttacccctgtattgggcaccaactccttcgttgtaccgg
agagcagcagttcggaccccactttcagcaatcctattcgattcccttttggattcacatggcctggtaccttttcccttatcattgaagcaatacatgc
ggattctgctgatgatttaaacacagagaaacccgaacgtctcattagtcggcttgccacccaacgtcaccttaccgttgggaaagcagtgggtcccaaa
atttgcacagcagtgaccggacagaactgaaatattcctaccgcttttgaatgtgatgaacactacttacggggaaagctggttctgactaactgccgtc
ccaaaaaaagaaggctttttggtccttttttccctgccgggggaaaaaaggagaaaaaaatttgtgcaaacccttggaaaggaaaggggccttgttatct
tgcccaaaaaaaccaatattttgtttttcccctggggaatgtgtaaattaa
3' EST: CF784916 [+]tttttttttttttttatttatagtatttatttntggaaaaatagtttaaaaattacttccttcattaacaaaaaaaaacagtagaaaaaaaatcaaatac
catttgcacaatattccataaatacatatatatacaaaaaaatatagtcacatagacccgaagtgcctttgtacatatttaccaaggtttaaattataaa
aatgaacatcacaaatacttaagctttacaaatatcgtaaaaatagtttcacaagttcaaaaaatattacgcattttttgacctagtagaaaacaccttt
tagtataaaaaagaagccattgaaggcagtgactgtctgtagtttagcaacggaatggctcaaacgaatccaaaatatacacctcagtcaggtcaccctt
cttctgacatatgttgaagtccattcattaaatatatatatatttatatatatatttattataataatttaaaaaaagcaactgtccatagtgcaatgga
gaagtacggaaacggcagtgcaacagtttatgtctggacacggagactagcttggggtgtccatattgttttgcagtctttttatttcccctaatttcag
tagagaaccagctcagcctgaatttagtgtctcagcagcagtcaacaaagggatatccccctgactctttcaccagcagcattcagatgggcgtcagctt
tgaatatctgacaaagaacttgntgctgtacagcgttccgtattacacttctgttggcatgatgcattcatcttttcccac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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