XB-CLONE-4351785
Clone Name: IMAGE:7027150
|
Gene:
myf5
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784672 [+]
taattaatcccccccccccccgagcacttagtcttgggcttttgtactttgtactttngacttggagaaagctgaccaaaggaactcctaagagatttcc
tgagacttgattgcttcaactccactgagcatctttacaagcagcagtattcagaatggagatggtagatacctgccatttttccccatctgaattcttc
tatgacagctcttgcattccttctccagaggagggatatacagaagactatgagcatggcatgtctctctatggagctcacaaaaaggatcttcaagaat
ccgatgaagatgagcatgtaagagcacccattggtcaccaccaagtcggtaactgtctgatgtgggcttgtaaagcctgcaaaagaaaatcttcctctat
gggatagaagggaaggttgcctcccctgagaagaaggggctaatggctctaataaacgtaaaatcagggcttttttggatactccttcataaatgaacgg
catccactgtatgagttccgcccagattcatgaggaatttgtctaattgggtggtgagaaattcctacaatataaaatctcctatgttaatatttagtga
gtatatatataggctccccctctataacgaacccgctaggtgttgctttagaaatattaaattgtgtcttaataaagaagatatgtgcggtgttaaaaag
ctctgcgtatctctcattgtgtgataaaaaggagattattttcatgtgtgtcactactagagatataacgtcctaaattatcaatgacattttacctccg
tctcttttgaagcttctatattcctcttgtgtcagctcagaatggtaaagaacgcgagtaaatataatggggggtttagatgtgggttttctttgcggcg
cacttcgggggaaaccgaaacgtaggtggctccacccttttcaacactttgtggacattatcccgccggtaaaaatgtggggtatatatagattatctaa
tacactctatatgtgccaaaatataaccaggatatcgtcatatgttatacttactcggccgagattttttagtctcagacaaaaaatcaatttcttttta
ttttcaatgatcatagtgtattatgtaatatttcttcatatcttgtttttatatcttctgaccccatgttttcactatagccgtataaatactcatttgt
caccacacatagtagggagatatagctgggaaaaatgttcgtttaatctgtcgccaccgcacccatttgctggtg
3' EST: CF784671 [+]tttttttttttttttcatagaaagaaaaaaacagcatgtttattaacacaatagcaattgggatttcttgctgtaggcttggttattaacgttaaataca
aaatgtagccaagtagaaatataatttaggcatttaaaaaataacaaaatgatttctttatatacatatctatacaaataataacataagtccttatacg
ccgatttgctggttgcaaggaaatgttttggattaagtcataatatttagcaagtatatgttaagggaattaacagaggtttatagtacatggtagattg
gtcggcaatcgggcatgtcaggtgatcgtggaaaggagtcagtgctactggtaggagacagggagagaagatctggaatagggaaggagcattgctcaga
agaggaaatccgatcccaagaactaaattaaacagtcaaaggctggacagaggtcattttagggtttgataaaaaactttgtcggtaaaaatccgaaccg
ataaacattgatccagagcggacagttgggttcaaaaacaatagagacgatgctggagaatagttcaggacacattgacgacacctagaaggtcggggct
tcaatggcatgcggggagaagctttgtatcctgagaagaatgggaagcaaattattatactgggtgccataacagacagggactatgtggaatcatctga
gagcaatatataaaaacgcgatagccttaacgaaggccatcccccgtttggagaacaccgggaattcgggggttttggggcctggcgtctcatactatca
gcaaagagttcctataagcatcgcgttattaaccttacgggaaagcagatctagccgt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: