XB-CLONE-4352088
Clone Name: IMAGE:7027268
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Gene:
abcb6
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784475 [+]
taattaatccccccccccccctttaaacctataattgccccccattctgcacacacaaggcacagcacccttgcccaccagtgaaaatggctcttcatac
ccattttcaccaaccagagcaatggctgagactgtgttatccaatgcagggcagtttgatttagaaatcactgtcctagttaccctttaaaaggcagcaa
agattttatacattaaagtgaaatgtataaataggtgtagaatctccatgaacagtacctgtacttgagagcagcgtcggactggccctcctagttaact
tttagtatgttacagaatggccagttctgtgccttcttcttctgacttattacaactttcaaatgggggtcactgaccccggcagacaaaaaaaactatt
gctctgtgaggctccaattttattgttagtgtaattttttataacttatttttctgttcaggtcctctcctatttatacaacagtctctcattcaagcca
ataatttcgacccaagctgctgaaattccaaactggagaactgctgaacaaaaaagggatataattaaaaaaaaacttgaaataaaaaaaatgaagacca
attgcaaattgtctcagactattactctctacattatgcaaaggtgaccaacccctttataaatggtatggctctcaggctgatggggattgtcctttgg
agaggggggtccattacagaaagattactggcaccctaataatccagtccgggccctgctcaagatgttttctatggggggggttcttggaaacagctga
ggcaataaac
3' EST: CF784474 [+]tttttttttttttttcatagaaaaagatcgtttatttgttatccaagaaaattcagtaataaatatggcggcacaaatgtaattaattacatccccatgt
atcctgcagctttggggagcaggttcatcagtcagtcagctgcaatcacatacattctgcctcacatttccaagacacccccatagaaacatcttgagca
gggcccgactggattattagggtgccagtaatctttctgtaataggaccccctctccaaaggacaatccccatcagcctgagagccataccatttataaa
ggggttggtcacctttgcataatgtagagagtaatagtctgagacaatttgcaattggtcttcattttttttatttcaagtttttttttaattatatccc
ttttttgttcagcagttctccagtttggaatttcagcagcttgggtcgaaattattggcttgaatgagagactgttgtataaataggagaggacctgaac
agaaaaataagttataaaaaattacactaacaataaaattggagcctcacagagcaatagttttttttgtctgccggggtcagtgacccccatttgaaag
ttgtaataagtcagaagaagaaggcacagaactggccattctgtaacatactaaaagttaactaggagggccagtccgacgctgctctcaagtacaggta
ctgttcatggagattctacacctatttatacattttcacttttaatgtataaaatctttgctgcctttttaaaggtaaactaggacagttgatttctaaa
tcaaactgcccctgcattgggataa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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