XB-CLONE-4352348
Clone Name: IMAGE:7027364
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Gene:
rpl7
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784308 [+]
taattaatcccccccccccccgctctttttccggtggtgaacatggcgggtacagaggagaagaaagtgccatctgtgccagaaagccttttaaaaaggc
gaaagcagtttgcagctgcaaagctcaagagagtcaaaaagatcgtggctgaaaaaaagcttcgcaaacagaagaggaagatcatttttaagagagctga
aggctactacaaggaatacaggcaaatgtacaggaaagaagttcgtctggccaggatggctcgtaaagctggaaacttttatgtacctgcagagcccaag
ctcgcttttgtgatcagaattagaggtattaacggagtcagccccaaggtacgcaaagtactgcaacttctccgcctgcgtcagatcttcaatggaacct
ttgtaaggcttaacaaggccagcataaacatgctgaggctggtggagccctatattgcatggggttaccccaatctgaagtctgtgcgccagctgatcta
caaacgtgggtaccttaagattaagaaccagcgtatccctctgactgacaattccctcattgagaaacaccttggcaagaaaggcgtcctgtgcgttgaa
gatctgatccatgaagtctacaccggtgggcaaaaacttcaaggcagcaaataaccttcctgtgggcccttccaacttgtccctctcccccgggggaggg
aattgaattaagaaaggaccacccccccttcttgttgggaaaggtgggctgaatgccccgggccaactaggggggaagaaaccgcggatataaacaccgg
ggctttcatttttacacaaggggagtggaaccctttaaagggtgtcttttcccccccgtggaaatggctct
3' EST: CF784307 [+]tttttttttttttttgttttaaaccagattttattaacagacagacttgaaggcatcatgggaaaacctttagttcatccttctaatgagcctgtttatc
tggtcttccctattgccagcatcaccaccttccacaaagtgtgttgtcttcttattcattcctcccctgggagaggacagtttgaagggccacaggaagt
tatttgctgccttgaagtttttgccaacggtgtagacttcatggatcagatcttcaacgcacatgacgcctttcttgccaaggtgtttctcaatgaggga
attgtcagtcagagggatacgctggttcttaatcttaaggtacccacgtttgtagatcagctggcgcacagacttcagatttgggtaaccccatgcaata
tagggctccaccagcctcagcatgtttatgctggccttgttaagccttacaaaggttccattgaagatctgacgcaggcggagaagttgcagtactttgc
gtaccttggggctgactccgttaatacctctaattctgatcacaaaagcgagcttgggctctgcaggtacataaaagtttccagctttacgagccatcct
ggccagacgaacttctttcctgtacatttgcctgtattccttgtagtagccttcagctctcttaaaaatgatcttcctcttctgtttgcgaagctttttt
tcagccacgatctttttgactctcttgagctttgcagctgcaaactgctttcgccttttttaaaagcttttctggcacagatggcactttcttctcctct
gntacccgccatgttcaccacggaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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