XB-CLONE-4352856
Clone Name: IMAGE:7026325
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Gene:
uba52
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077658 [+]
CTTTTCTAATCAGTCGGCCGCCGTGCGGTAAACGGGAAGATGCAAATCTTTGTTAAAACCCTTACTGGGAAAACAATCACCCTTGAGGTTGAGCCCAGTG
ATACTATTGAAAATGTGAAGGCCAAAATCCAGGACAAGGAAGGTATTCCCCCTGACCAACAGCGTCTGATCTTTGCTGGTAAACAACTTGAAGATGGACG
CACCCTATCTGATTATAACATCCAGAAAGAGTCCACTTTGCATCTGGTGTTGCGTCTGCGTGGTGGTATCATTGAGCCTTCCCTTCGCCAACTTGCTCAG
AAGTACAACTGTGACAAAATGATTTGCCGCAAGTGCTATGCCCGCCTTCACCCTCGTGCTGTAAATTGCCGTAAGAAGAAGTGTGGTCACACCAATAACC
TGCGTCCCAAGAAGAAGGTCAAATAAACTGACCTTTGCATCTATTTTGATTGTTGATAACAAATAAAAAAAAAACAGAAGACAAAAAAAAAAAAAAAA
5' EST: CF783983 [+]taattaatccccccccccccccttttctaatcagtcggccgccgtgcggtaaacgggaagatgcaaatctttgttaaaacccttactgggaaaacaatca
cccttgaggttgagcccagtgatactattgaaaatgtgaaggccaaaatccaggacaaggaaggtattccccctgaccaacagcgtctgatctttgctgg
taaacaacttgaagatggacgcaccctatctgattataacatccagaaagagtccactttgcatctggtgttgcgtctgcgtggtggtatcattgagcct
tcccttcgccaacttgctcagaagtacaactgtgacaaaatgatttgccgcaagtgctatgcccgccttcaccctcgtgctgtaaattgccgtaagaaga
agtgtggtcacaccaataacctgcgtcccaagaagaaggtcaaataaactgacctttgcatctattttgattgttgataaccaataaaaaaaaaacggaa
gaacaaaaagaaaaaaaaagggatcccctagttctagaagcggccgccacccccggtgggaactttgggccctggcccaaacttgttttttttgcggcct
ttataattgggttacaaaataagagccaataagcattcccaaaattttccccaaaataaaagcatttttttttccacgggcatttctaaatttgggggtt
ttggtccaaaactccatccaaaggtatccttaatcataggtcctggggatccgggaaatttaatttggggcgccacacaccattgggcctggaaaaataa
cctttggaaaaaaaggaaactttggttttaaggaacctcttctagaaggcgggaaaagaaacccctcctttgggaaatttgggggtccagtttagg
3' EST: CF783984 [+]tttttttttttttttgtcttctgttttttttttatttgttatcaacaatcaaaatagatgcaaaggtcagtttatttgaccttcttcttgggacgcaggt
tattggtgtgaccacacttcttcttacggcaatttacagcacgagggtgaaggcgggcatagcacttgcggcaaatcattttgtcacagttgtacttctg
agcaagttggcgaagggaaggctcaatgataccaccacgcagacgcaacaccagatgcaaagtggactctttctggatgttataatcagatagggtgcgt
ccatcttcaagttgtttaccagcaaagatcagacgctgttggtcagggggaataccttccttgtcctggattttggccttcacattttcaatagtatcac
tgggctcaacctcaagggtgattgttttcccagtaagggttttaacaaagatttgcatcttcccgtttaccgcacggcggccgactgattagaaaagggg
ggggggggggattaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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