XB-CLONE-4352976
Clone Name: IMAGE:7026370
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Gene:
ube2b
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077659 [+]
GGAGCAACGTCATTGCGGCTTGTTTGTGCCAGGGTTGCTGAGTTAGCGGCGGCCGGGTTTGGGCTCCCTCCCTTCCCCCTCCGTACTGCTTCTATTCAGG
GGCAATGTCCACCCCGGCCAGGAGGAGGCTGATGAGGGACTTCAAGAGATTGCAGGAAGACCCACCAGTTGGAGTCAGTGGCGCACCTTCAGAAAACAAT
ATAATGGTGTGGAATGCAGTTATATTTGGGCCTGAAGGGACACCATTTGAAGATGGTACATTTAAACTTGTCATAGAATTTTCAGAAGAGTATCCGAATA
AACCTCCAACTGTTAGATTTGTATCAAAAATGTTTCACCCAAATGTTTATGCTGATGGCAGCATATGTTTAGATATCCTTCAAAATCGCTGGAGTCCAAC
ATATGATGTATCATCAATATTAACTTCAATTCAGTCTCTTCTGGATGAACCAAACCCAAATAGCCCTGCCAACAGCCAAGCAGCGCAACTGTATCAAGAA
AACAAGCGAGAGTATGAGAAAAGGGTATCTGCAATCGTTGAGCAAAGTTGGAATGACTCCTAACAGATCACTGCGTTTCATCCCTTTCGTTTTTTTTGTG
TTTGATTTAAAATTCGTAAAAGACAAATAAGGAGGCATCTGTAGATCATCGCACTAGTTTTAAGGAGAAAGAGTCGTCCTTAAACACAAAATCTTATTTC
CCCCTCACCTTATTTGGAAAATTTCAAGGCTTTCTCTACTAATGTACTTTTTATTGCATGGTATGCATGATATCAAATAAGTTATTGCTATATTTGTAAT
ATAACCTATTTGTATTTTTTCCCTCAATGTATAATGTTGCTGTGAAGATTTCATGGAAAAAATATACACATTTTGTAAATCTGTAACTTTTTCTCCCCAT
CCCCCACAAAATGGAATGTTTTTGTTTTTTTTGGCAGCAGCAAAACTGCAGGTTTTTTTTTTTTTTTTAAAGTATTTTAAAGTAAACTATTCAATGAATA
GCTCACATAAAAAAAAAAAAAAAA
5' EST: CF783907 [+]taattaatcccccccccccccggagcaacgtcattgcggcttgtttgtgccagggttgctgagttagcggcggccgggtttgggctccctcccttccccc
tccgtactgcttctattcaggggcaatgtccaccccggccaggaggaggctgatgagggacttcaagagattgcaggaagacccaccagttggagtcagt
ggcgcaccttcagaaaacaatataatggtgtggaatgcagttatatttgggcctgaagggacaccatttgaagatggtacatttaaacttgtcatagaat
tttcagaagagtatccgaataaacctccaactgttagatttgtatcaaaaatgtttcacccaaatgtttatgctgatggcagcatatgtttagatatcct
tcaaaatcgctggagtccaacatatgatgtatcatcaatattaacttcaattcagtctcttctggatgaaccaaacccaaatagccctgccaacagccaa
gcagcgcaactgtatcaagaaaacaagcgagagtatgagaaaagggtatctgcaatcgttgagcaaagttggaatgactcctaacagatcactgcgtttc
atccctttcgttttttttgtgtctgatttaaattcgtaaaagacaaataaggaggcatctgtagatcatcgcactagttttaaggagaaagagtcgtcct
tnaacacaaaatcttatttccccctcaccttnatttggaaaatttcaagggctttctctactaatgtacttttttattgcatggngtatgcatgatattc
aaataagttatttggctataatttgtaataaa
3' EST: CF783906 [+]tttttttttttttttatgtgagctattcattgaatagtttactttaaaatactttaaaaaaaaaaaaaaaacctgcagttttgctgctgccaaaaaaaac
aaaaacattccattttgtgggggatggggagaaaaagttacagatttacaaaatgtgtatattttttccatgaaatcttcacagcaacattatacattga
gggaaaaaatacaaataggttatattacaaatatagcaataacttatttgatatcatgcataccatgcaataaaaagtacattagtagagaaagccttga
aattttccaaataaggtgagggggaaataagattttgtgtttaaggacgactctttctccttaaaactagtgcgatgatctacagatgcctccttatttg
tcttttacgaattttaaatcagacacaaaaaaaacgaaagggatgaaacgcagtgatctgttaggagtcattccaactttgctcaacgattgcagatacc
cttttctcatactctcgcttgttttcttgatacagttgcgctgcttggctgttggcagggctatnttgggtttggttcatccagaagagactgaattgaa
gttaatattgatgatacatcatatgttggactccagcgattttgaaggatatctaaacatatgctgccatcagcataaacatttgggtgaaacatttttg
atacaaatctaaacagttggaggtttattcggatactctttctgaaatttctatgacaagtttaaatgtaccatccttccaaatggtgtccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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