XB-CLONE-4353062
Clone Name: IMAGE:7026404
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Gene:
b3gnt5
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080164 [+]
TGTGTGAGCCAGGAAGCGGACTTGCCAGCGCGTTCATCGCCTGCTGTAGCTGAGCGCATCTATGCCGCCTGCCTGGAATCATTTCTGTGTGTCGATGATG
TCAGCTAACTAAGACACAGTAGAAGCATGTGCCATTTTTGAAGTCTTCCTGGAACCGTCAGCAATGCAAACGTTGAAAATCTGTTAGCCTACAGTGTGGG
TTTTTCCATTTTAAGGAGTATTTGGCTTTTACAATGAACATTTGTTCAGGAAATACAGACATTCAAAGACAAGCTATATGAATTTCAACCTTTTTTTCCT
CACAAAATTTCTAATTCCCCCAGAATGCTTATTAGTGCTAGGAGGCTAAGAAGATGCCAGTTCTTTCAATTACTCACTTCATGTTTTGTTTTATCATTAA
TGGCTTTGTTGGTTCAAGAAGACAATTCCTTGATCAACCACGTAAAGTCGTATTCCTACAGATACCTCATAAATAGCTATGACTTTGTCAATGATAGCCT
GTCTGTTCCTCGGGACAGACCCGATGGGGCACCCAGCTACAGATATCTGATCAATAACCGAGACAAATGTCAAAACGAGGATGTCCTTCTATTACTGTTT
GTAAAGACATCTCCTGAAAACAGAAGGCGCCGCAATGCAATTCGAAAGACTTGGGGCAACGAGGATTACATTCGTTCTCAGTATGCTGCCAACATTAAAG
TTGTGTTTGCCCTTGGTATTGAAGCAGATCCAGTAAAAAGTCATCAAACTCAAAAAGATCTTGTCATTGAAAACAAAAGATTCAACGATTTAATACAGCA
AGACTTCAAGGACACTTTTCACAACCTAACTCTTAAGTTGCTTTTGCAGTTTGGGTGGGTAAATTCATACTGTCCTAGTGCTAAATTCATTATGTCAGCC
GATGATGATATCTTTGTCCACACACCTAACTTGGTTTCCTACCTTAAAAGCTTGCCTATCGAAACACAGGATTTCTGGATTGGTAGAGTCCATCGTGGAT
CACCTCCAATAAGAAGCAAAACCAGCAAGTATTATGTGCCTTACGAAATGTATCCCTGGTCATCTTATCCCGACTACACTGCAGGGGCTGCTTATGTTGT
TTCTAAAGATGTAGCTGCAAAAGTATACGAAGCGTCACAGACATTAAATACCAGTTTATACATAGATGATGTTTTCATGGGAATCTGTGCCAACAAAATG
GGAGTTGTGCCTCAATATCACGTTTATTTCGCTGGAGAAGGAAAAGCCCCATACCATCCTTGTATTTACAACAAAATGATTACTTCCCATGGACATTTAG
ATGACCTTGACTATTTATGGAGGCAAGCTACAGACCCTAATGTAAAATCTCTTAGTGCTGGAGTTTTGGGAGGTGCATACTGTAAGTTAGTAAACATCAT
GCTTCTCTGTAAACTTAGCTATGTGGATACATACCCCTGTTCAGCCGCATGGTCCTAGTGCTACTGCAATAGTTGGGCTTGCTTTCAGTTTGATTTTTTG
GCAAACATTCTTTGGATCCTTTGTGGTCCCCACCCATCTGCTCTCAGGAACACATACTATTTTTGCTTTTGATGACGCTTTTGAAGTCCGTTACTGAACA
ACAGTAATAAATGCTGAAATGTTTACTGTGATGCACTGTATCACTCATGCTGTAATACGTTAATGGCTGAATTCTATGTTCTTTTCATGTCCTTGTTTAC
AGATTTGTTGTATGATATAGCAGTGATGTTGTTAAACCATGTACAAGTATAAAGCATAAAGAAATCCCTATGAGAATACAGCACAGTATAAGTTTAGAGA
AGCCAGATTGGCTTGCTATGGATGTACTGCACTGTCTAAAGCTTTAGCAGTTGAGGGATGCTGGGAAGTTTAGTTTGCAACAGCTGGAGAAGAACCATAT
GGGAATCCTTGGTCTCAATCTGAAGCTGTGGGCTGTTACTGTGCCTTCTAATGTCCAGCTAAAAAAACCAAAATTATAAGTGACTAAGAGTTTTTGTATT
TATGCATGTTATAGGTTGGCTGTTGTGGATTTTATCATCCAAAAAGCTGCATTTACAGCAGGTTTTAAACTTAGTCATAAAATGATAACCAGTTATTCAT
AGGTTATTTACTCTTTCCACCTTTAGGATCACTGCATTAAATCAGGGGTGGGTAATCTGCAGCTTTCCTAATGCTGTTGGACTGCAGTTTTTACATCCCA
CTGGTAGTGAGGCAGAAGAATGCCCTTGTGTTTTATTTAAATACTGGCGCTTTATAGTATAGATTAAACCACTTGGTATTTTAGCACAGTCACAGGCAGG
GTTCTATATAAATAAAGCTCACTAGAATTAGAAGAACTTGCATACTAAAAAAAAATGTGAAAAAATTCAGAGTGAACATATTTTGAACTACAGCAGGTCT
GTTTTACACACAAATATATTTTTATACTGTATTAGAACTGCATATTTATTATAAAAGATATGAAGGTGGGAAAAACAAACCACCTGAATTTTCATACAGA
GGAAATCCTTTTGATAACTCGATTTTCTTGTGGAGGGATTCCCAACTAAAATGGTGCTGGTTATTGGAAGTGTGAGGTGGAGAAATCCTTTTCAGTTGTA
CATCAGAGCCTTTCAGTGGTTCTAAACTAGTGAGCTGTGGATGAGTGAAAGACACGTGCACTTGTGTATTTATTGTCTGCCGTTAACTGTATGTTTTGTG
TTTTTTTTTATGTTGTAAATTATTGCTTTGCTCGTATGAGCAATTTTACTTTTGTAAATCTTTTCATTTTCAATAAACTGACTCCATAATAAAAAAAAAA
AAAAAA
5' EST: CF783852 [+]taattaatccccccccccccctgtgtgagccaggaagcggacttgccagcgcgttcatcgcctgctgtagctgagcgcatctatgccgcctgcctggaat
catttctgtgtgtcgatgatgtcagctaactaagacacagtagaagcatgtgccatttttgaagtcttcctggaaccgtcagcaatgcaaacgttgaaaa
tctgttagcctacagtgtgggtttttccattttaaggagtatttggcttttacaatgaacatttgttcaggaaatacagacattcaaagacaagctatat
gaatttcaacctttttttcctcacaaaatttctaattcccccagaatgcttattagtgctaggaggctaagaagatgccagttctttcaattactcactt
catgttttgttttatcattaatggctttgttggttcaagaagacaattccttgatcaaccacgtaaagtcgtattcctacagatacctcataaatagcta
tgactttgtcaatgatagcctgtctgttcctcgggacagacccgatggggcacccagctacagatatctgatcaataaccgagacaaatgtcaaaacgag
gatgtccttctattactgtttgtaaagacatctcctganaacagaaggcgccgcaatgcaattcgaaagactttggggcaacgaagattacattcgttct
cagtatgctgcccaacattaaaagttgtgttttgcccttggtattggaagcaaatcccgttaaaaagtcatcaaacctcaaaaagatccttgtcattgag
aaacaaaaagattccacgaatttaaatacaaccaggaacttcaagggaacccctttttaccaaccctaacctccttaagtttggcttttttgccaaattg
tggggggggggtaaaaaatccaaaaacggggccccaagagt
3' EST: CF783851 [+]tttttttttttttttattatggagtcagtttattgaaaatgaaaagatttacaaaagtaaaattgctcatacgagcaaagcaataatttacaacataaaa
aaaaacacaaaacatacagttaacggcagacaataaatacacaagtgcacgtgtctttcactcatccacagctcactagtttagaaccactgaaaggctc
tgatgtacaactgaaaaggatttctccacctcacacttccaataaccagcaccattttagttgggaatccctccacaagaaaatcgagttatcaaaagga
tttcctctgtatgaaaattcaggtggtttgtttttcccaccttcatatcttttataataaatatgcagttctaatacagtataaaaatatatttgtgtgt
aaaacagacctgctgtagttcaaaatatgttcactctgaattttttcacatttttttttagtatgcaagttcttctaattctagtgagctttatttatat
agaaccctgcctgtgactgtgctaaaataccaagtggtttaatctatactataaagcgccagtatttaaataaaacacaagggcattcttctgcctcact
accagtgggatgtaaaaactgcagtccaacagcattaggaaagctgcagattacccacccctgatttaatgcagtgatcctaaaggtggaaagagtaaat
aacctatgaataactggttatcattttatgactaagtttaaaacctgctgtaaaatgcagccttttttggatgaaaaaaaatccacaacagcgcaaccca
ataaccaggcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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