XB-CLONE-4353490
Clone Name: IMAGE:7026189
|
Gene:
ptmap12
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF783580 [+]
taattaatcccccccccccccccattcctctccactgagtcgtgcggctgctcggtgactttgttccatccattcgtcacattttccccgctccgaaacc
aaagaggtctttattttttcctcttttcccgaattaagaaactctcaaaggaaaatggcagataccgacgtagataccaccaccgccgaggtccccgtca
ccaaggatcttaaagaaaagaaagaagtggtagaggaaccagagaaggcagagaatggcaagggagatgccccatctaatggcacagaagagaatggcgc
tgatcatggtgcacagaatgatgatgcagaggaagaggatgaagaaggggatgtagaaggtgagggtgagggtgaaggagaggaagaagaggatgaggaa
gaaggtgaaggagatgaaaatgagacagatggtctttcagtaaaacgtcctgcggaagatgaggagaaaactgaaactaaaaaacagaagacagaaaatg
gagactccacagaagtgaaagaatctgcctaaattctccttccagacattttatagcttaagtgacatggttgactttaaattgtagagaatgtgcttta
tatctgtacattttacaaaatgactttttatatactgtaaacttacatgcactagaaacctgtaacatcacagcaagatagctaaggttttttgtaaaat
gttatttattggtgcttgtttgtactggttaactgctttcaataaaaatctaaagttttttttttttttggtttaagtggtacagctttgctttttgtac
accattggaagcccatg
3' EST: CF783581 [+]tttttttttttttttatataaaagatttatttatagtacgtaacctgaatatacaaagtagtggtaagcataaactaatgaggtatacagtttttatctt
ggttataactgatgaaaactacactgtcaatgcctcaaaatttctgcagaacacacacactttgatagcacattaggatacagagaaatttacatattga
accttgcaattgtcaagccttgcttgcaccacaactatataccaaacagcaacaaaattaagcccaaagatctatacagaattcccaggttaaccctggc
taaataagatccacatctttggccttcattgcatatgaactttctagcctaccaccattatattacaggaaggcagcttcactcaagaatacacatggct
gtactaatatgataagacatgtgcttcaatggtgtacaaaagcaaagctgtaccacttaaacaaaaaaaaaaaaaactttagatttttattgaagcagtt
aaccagtacaaacaagcaccaataaataacattttacaaaaaaccttagctatcttgctgtgatgttacaggtttctagtgcatgtaagtttacagtata
taaaaagtcattttgtaaaatgtacagatataaagcacattctctacaatttaaagtcaaccatgtcacttaagctataaaatgtctggaaggagaattt
aggcagattctttcacttctgtggagtctccattttctgtcttcgggtttttaatttcagtttttctcctcatctttccgcagggactttttactggaaa
gaccatcttgtctt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: