XB-CLONE-4353607
Clone Name: IMAGE:7026234
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Gene:
rpl7
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783507 [+]
taattaatcccccccccccccctctttttccggtggtgaacatggcgggtacagaggagaagaaagtgccatctgtgccagaaagccttttaaaaaggcg
aaagcagtttgcagctgcaaagctcaagagagtcaaaaagatcgtggctgaaaaaaagcttcgcaaacagaagaggaagatcatttttaagagagctgaa
ggctactacaaggaatacaggcaaatgtacaggaaagaagttcgtctggccaggatggctcgtaaagctggaaacttttatgtacctgcagagcccaagc
tcgcttttgtgatcagaattagaggtattaacggagtcagccccaaggtacgcaaagtactgcaacttctccgcctgcgtcagatcttcaatggaacctt
tgtaaggcttaacaaggccagcataaacatgctgaggctggtggagccctatattgcatggggttacccaaatctgaagtctgtgcgccagttgatctac
aaacgtgggtaccttaagattaagaaccagcgtatccctctgactgacaattccctcattgagaaacaccttggcaagaaaggcgtcatgtgcgttgaag
atctgatccatgaagtctacaccgtttggcaaaaacttcaaagcagcaaataactttcctgtggccctttcaaactgtccctctcccagggggaggaatg
gaataacgaagaccacacccctttgttggaaacgtgggtgaatgctgggcaaataggggaagtacccagaataaaacagggcttcatttagaaaaggaag
ggaacacttaaaggggttttttccccactggaaggtgccttttcccaggtccggggtccgtggttaaaa
3' EST: CF783506 [+]tttttttttttttttattttttttttttttttttttaattgttttaaaccagattttattaacagacagacttgaaggcatcatgggaaaacctttagtt
catccttctaatgagcctgtttatctggtcttccctattgccagcatcaccaccttccacaaagtgtgttgtcttcttattcattcctcccctgggagag
gacagtttgaagggccacaggaagttatttgctgccttgaagtttttgccaacggtgtagacttcatggatcagatcttcaacgcacatgacgcctttct
tgccaaggtgtttctcaatgagggaattgtcagtcagagggatacgctggttcttaatcttaaggtacccacgtttgtagatcaactggcgcacagactt
cagatttgggtaaccccatgcaatatagggctccaccagcctcagcatgtttatgctggccttgttaagccttacaaaggttccattgaagatctgacgc
aggcggagaagttgcagtactttgcgtaccttggggctgactccgttaatacctctaattctgatcacaaaagcgagcttgggctctgcaggtacataaa
agtttccagctttacgagccatcctggccagacgaacttctttcctgtacatttgcctgtattccttgtagtagcctttagctctcttaaaaatgatctt
ccccttctgtttgcgaaacttttttttcagccacgatctttttgactcttcttgagct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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