XB-CLONE-4353895
Clone Name: IMAGE:7026439
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Gene:
ptmap12
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF723055 [+]
taattaatccccccccccccccggtttcattcctctccactgagtcgtgcggctgctcggtgactttgttccatccattcgtcacattttccccgctccg
aaaccaaagaggtctttattttttcctcttttcccgaattaagaaactctcaaaggaaaatggcagataccgacgtagataccaccaccgccgaggtccc
cgtcaccaaggatcttaaagaaaagaaagaagtggtagaggaaccagagaaggcagagaatggcaagggagatgccccatctaatggcacagaagagaat
ggcgctgatcatggtgcacagaatgatgatgcagaggaagaggatgaagaaggggatgtagaaggtgagggtgagggtgaaggagaggaagaagaggatg
aggaagaaggtgaaggagatgaaaatgagacagatggtctttcagtaaaacgtcctgcggaagatgaggagaaaactgaaactaaaaaacagaagacaga
aaatggagactccacagaagtgaaagaatctgcctaaattctccttccagacattttatagcttaagtgacatggttgactttaaattgtagagaatgtg
ctttatatctgtacattttacaaaatgactttttatatactgtaaacttacatgcactagaaacctgtaacatcacagcaagatagctaaggtttttttg
taaaatgttatttattgggtgcttgttttgtactggtttaactgcttcaataaaaaatctaaaagttttttttttttttttggtttaaagtgggtacaag
ccttttgcttttttgtaacaccaattggaaagcccaa
3' EST: CF783323 [+]tttttttttttttttatataaaagatttatttatagtacgtaacctgaatatacaaagtagtggtaagcataaactaatgaggtatacagtttttatctt
ggttataactgatgaaaactacactgtcaatgcctcaaaatttctgcagaacacacacactttgatagcacattaggatacagagaaatttacatattga
accttgcaattgtcaagccttgcttgcaccacaactatattacaaacagcaacaaaattaagcccaaagatctatacggaattcccaggtttaacctggc
taaaaaagaatccacactctttggcccttcattgcctaagaaacctttctagcccaacccccccttatattttccggggaagggcacccttccctccaaa
aaataaacccatggggcttggttcctcaattatgtaaaaaaacacctgtgggcccctcacaagggggggggtacaaaaaaacccaaaaattctttgtccc
cccctttttgaacccctaaaagaaaataaaaaaacaacccttctaagagactttttcctttgtgggagcgagttttttaaaccccatgtgaaaaaaaata
aggcgcctcctatgaagaaaaattataaacttttttttaaaaaagaaaaaccccctttcgtcccctcaccctttcttttccgccgctatagcgtcaacca
agggtgtgtttcttatagggggggtaggtgaacacactttttattttcttgcgcgttttacctaaaatacccgccccccttctttttgattacaaaccgc
cttttcgacacatcaaataaataagtcccccctacttttttccctcaaagaaactttttttgaccgccattttaatacgggtgcggcgccgctcccttta
cccccttccacataaaaaactattcgcttttcgttggacgctgttaaaacccatttcaccttccgccggtcgatatatatttct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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