XB-CLONE-4357475
Clone Name: IMAGE:7029266
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Gene:
neurod4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084160 [+]
GAGCCCCGGACTCATTGATATTGGGCACAGCGAGTCTGCCTGGGAGCTGTCCAGCACTCCATGCTCCTGAAATAACTTGGGCAACAAGTCCGATCTGCCC
GCTACTCTGTGCCTCCAGCTCAGGCCCGGGGAGAGGGACCCTGCTGAGCAGGACTCAGGACACTGTTTGAAGATCACATCAAATTCTGCTAATATGTCGG
AGATAGTCAGTGTGCATGGGTGGATGGAGGAAGCCCTTAGTTCCCAGGATGAGATGGAGAGGAATCAGCGGCAATCTGCCTATGATATCATTTCAGGTCT
GAGTCACGAGGAAAGGTGTAGCATAGATGGAGAAGATGATGATGAAGAAGAAGAGGATGGAGAGAAACCAAAAAAGAGGGGACCCAAAAAAAAAGAAGAT
GACCAAGGCTAGACTGGAGAGGTTTCGTGTGCGCAGAGTAAAAGCCAATGCCAGGGAGCGCACCAGAATGCATGGACTTAATGATGCCCTAGAAAATTTA
AGGAGGGTCATGCCTTGCTATTCCAAAACACAAAAGTTGTCTAAAATTGAGACCCTTAGACTGGCCAGAAACTATATATGGGCATTATCTGATATTCTAG
AACAAGGTCAAAGTACAGAGGGAAAGGGCTTTCTGGAAATGCTCTGCAAAGGTCTTTCTCAGCCAACAAGCAACTTAGTAGCTGGCTGTATGCAACTTGG
ACCTCAGGCAATGTTCTTGGATAAACACGAAGAAAAGTCTCATTTATGTGACTCTTCTCTTACTGGTCATACCTATAATTACCAGTCCCCAGGACTACCC
AGTCCACCTTATGGAAACATTGATGTTCACCATTTGCACTTGAAACCCCCTTCTTTTAAACCAGTAATGGATCCATCTGTGGTAAGCCATACACTAAACT
GCACCACTCCACCCTATGAAGGAGCTTTGACACCCCCGCTCAGCATCAGTGGTAATTTTTCCTTGAAGCAAGATGGTTCACCTGATATGGACAAATCATA
TGCATTTAGGTCCCCCTACCCAGCTCTTGGGCTTAGTGGATCTCATGGACATGGGTCACACTTTCAAACCGGTGTCCCAAGGTATGAACTACCCATAGAA
ATGGCTTATGAGCCCTACCAACACCATGCTATATTCACTGAATAAATGTGCTGATCAAATGACTTTTTATAGTTGACCCAAACCTGACTTTTTTGGATGA
CAATGGCCACCACATCACCACCACCTAAGATGACTGTTGGTCTTATTGTACGTATGTTTTGTAGTTTGTTTGTAGGTCACTTTTGAGACTCTTGGAGCCT
GAATATAATGCATAGAAAAATCCAGTCAATCACAATGATAGCTACAAATTGTAATGTGAAAACAAAACACTAATGTGAACATTGCTATTTGGTGGAAACG
TACCAGCACATACATATAGCCACATATAGTAACATATAATAACAGGAACTCATCTCAGGGGAACAACACTGGGGAGTGCATTGCTCCTCTAAACACCAAG
GTTTCCAAATGGTACACACAACATACTGACCCAATGGCAGAGGAGTGTGTTAGTTGTTACTGGTTACTAGCACTCATTTTTACCCCTAAACTGCTCATAG
AAGATAAAAATTTACAATGTATGCAAGGACCGATTTAAAGCACAAGTTTATTATGTATCAATTGCACTACAATATCCAATGCTATAAACTACAGTATAGC
TGTTTTTTATTTTTTATATTACTGCTATATCACACAGAGCCATACGGTACATGCATATTTAATACAATGTGTATATTTTTTGTTGAGAATCCCATCGCCT
ACATATCATGAAATACAAAAGGCCAAAAACCCTTCTAACTATTATTTATACCTTTTAATTTATTTTATTATTATTTTGTATTACTGCATTTGTCAAATGA
TCCTACAACGGAATGCTCTTACTGTTCATGAATGATTGTTTTTGTATTTGTTGGTTTCAAACTGCATCCTTATTTATATCATTATTTATTTTAATAGAAT
TTTTAAATTAATCTGTTGCTCAAAAAAAAAAAAAAA
5' EST: CF781447 [+]tattaatcccccccccccccgagccccggactcattgatattgggcacagcgagtctgcctgggagctgtccagcactccatgctcctgaaataacttgg
gcaacaagtccgatctgcccgctactctgtgcctccagctcaggcccggggagagggaccctgctgagcaggactcaggacactgtttgaagatcacatc
aaattctgctaatatgtcggagatagtcagtgtgcatgggtggatggaggaagcccttagttcccaggatgagatggagaggaatcagcggcaatctgcc
tatgatatcatttcaggtctgagtcacgaggaaaggtgtagcatagatggagaagatgatgatgaagaagaagaggatggagagaaaccaaaaaagaggg
gacccaaaaaaaaagaagatgaccaaggctagactggagaggtttcgtgtgcgcagagtaaaagccaatgccagggagcgcaccagaatgcatggactta
atgatgccctagaaaatttaaggaggggcatgcccttgctattccaaaacacaaaagttgtctaaaattgagacccttagactgggccagaaaactatat
atggggcattatccgaatattctagaaccagggtcaaagtaccgaaggaaaagggcctttctgggaaattgcctctgcaaagggtttttcttcacccaac
cagtcaactttataaacctgggctgtatgccaaatttgggaccctcggggcaatggtccttggaataaaacccaatagaaaaagccctcattttaatgtg
aactccttacacct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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