XB-CLONE-4358037
Clone Name: IMAGE:7028380
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Gene:
lap3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084523 [+]
CCCAGAGACAGCTGAATAGCAGGCGTGATGCTGCCTTTCAGAACTTTGCTCAAATGGAGCGTTAATAGGAACTGCTGCAGGGGGTTCGCTGTGTCACAAC
AGAACTACAATAGTGTGAAAAAGGGTCTTGTCCTTGGAGTGTATGAGAAAGAAAAGGAAGAAGAATCGCTCACTCTGACAAATGCAGGAGATGCTGTTGA
TAATGCTGTTTTAGGAAAGCTGCGTGACCAGTTGGCCAGATCAGGACCATCTCTCAAAAAAGGCAAATCACGAATATTTTATGGGTTGCATGAGGATTTT
CCAAGCATTGTTGTGGTTGGCCTTGGCAAGAAATCTGCAGGAGTCAATCAACATGAACTCTGGAATGAAGCCAAGGAGAATATTAGAGCAGCAGTGTCAG
TGGGCTGCAGGCAGATGCAAGATATGGAGATTGTGCAGGTAGAAGTGGACCCATGTGGAGATGCCCAGGCTGCAGCTGAAGGGGCTGTCCTTGGACTGTT
TGAATACAATGAGATGAAGAAGAAAAAAAAGAAAGCAGTTACCACTCATCTTCATGGAAGCAGCGAGATCACTGCCTGGGAGAAGGGAGTGCTGTACGCT
GAGGGGCAAAACCTGGCTCGCCATTTGATGGAAGCGCCGGCCAATTACATTACACCTACAAAATTTGCAGAGACATTTGAACAGAGGTTGGCGAATATGG
GCAGTAATGTTAAGGTGTTTACCAGATCAAAACAGTGGATAGAAGAACAACAAATGGGGGCATTCCTAAGTGTGGCCAAAGGGTCTGAAGAGCCCCCGGT
CTTCCTGGAAATTCATTACAGCGGCAGCTCTGATGCAAGCCAGCCTCCTCTAGTATTTGTTGGAAAAGGAGTGACATTTGACAGTGGTGGAATTTCTCTG
AAACCTTCTTCAGGCATGGATGCAATGAGAGGCGATATGGGGGGAGCTGCTACAGTTTGTTCTGCCATCACGACTGCTGCAAAGCTAAAGTTACCTATCA
ATATTATCAGTCTAGCTCCATTGTGTGAAAATATGCCAAATGGACGAGCAAATAAACCCGGTGATGTGGTCAAGGCCAAGAATGGAAAAACAATTCAGGT
TGATAATACTGATGCAGAAGGAAGGCTTCTTTTAGCTGATGCTCTCTGCTACGCACACAGTTTTAATCCTCGGGCAATCGTTAATGCTGCAACATTAACA
GGTGCAATGGATGTCGCATTAGGATCTGCTGCAGCTGGAGTATTCACAAACTCTTCCTGGCTTTGGACTCACCTTCAGGAAGCCAGCGTTGTTACCGGAG
ACCGTGTATGGAGAATGCCACTGTTTGAGCACTACAGCAAACAAGTGACTGAATCAGCTCTTGCTGACCTGAACAATATTGGAAAATACAGCAGATCGGG
AGGAGCCTGCACAGCTGCCGCCTTCCTGAAAGAGTTTGTAACTGCACCTCACTGGGCTCATCTGGACATTGCCGGCGTGATGTCAAATAAGGATGAGGTG
CCGTACCTTCGCAAAGGCATGTCCGGCCGCCCCACGAGGACATTAATAGAGTTTGCTGCCCGTCTCAGTGAAGACAAACAAACTATTTAATGAAACATCT
CTGTTTTATCTTGAAACCACACAGAATTATTTCTATTATTAAATTTGCCAGAAAACCTGAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAA
5' EST: CF781166 [+]taattaatccccccccccccccccagagacagctgaatagcaggcgtgatgctgcctttcagaactttgctcaaatggagcgttaataggaactgctgca
gggggttcgctgtgtcacaacagaactacaatagtgtgaaaaagggtcttgtccttggagtgtatgagaaagaaaaggaagaagaatcgctcactctgac
aaatgcaggagatgctgttgataatgctgttttaggaaagctgcgtgaccagttggccagatcaggaccatctctcaaaaaaggcaaatcacgaatattt
tatgggttgcatgaggattttccaagcattgttgtggttggccttggcaagaaatctgcaggagtcaatcaacatgaactctggaatgaagccaaggaga
atattagagcagcagtgtcagtgggctgcaggcagatgcaagatatggagattgtgcaggtagaagtggacccatgtggagatgcccaggctgcagctga
aggggctgtccttggactgtttgaatacaatgagatgaagaagaaaaaaaagaaagcagttaccactcatcttcatggaagcagcgagatcactgcctgg
gagaagggagtgctgtacgctgaggggcaaaacctggctcgccatttgatggaagcgccgggcaattacattacacctacaaaatttgcagaaacatttg
aacagaaggttggcgaatatgggcagtaatgttaaggtgttttaccagatcaaaaacagtggaaaagagaaaccaacaaatggggggcattcccttaagt
ggtggccaaagggttctgaaaaaaacccccgggtcttccctggaaaatttttttaaagcgggcagcctctttaatgcaaagccacgccctcccctcctag
taatttgt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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