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GEO Series: GSE111454

Title: RNA Sequencing facilitates the identification of transcriptional targets of Pitx1 in Xenopus laevis

Summary

The cement gland in Xenopus laevis has long been used as a model to study the interplay of cell signaling and transcription factors during embryogenesis. The homeodomain-containing transcription factor Pitx1 has been linked to cement gland development. However, the downstream transcriptional targets of Pitx1 remain unknown. Here, we utilize RNA Sequencing to identify transcripts whose expression are affected by Pitx1 misexpression in animal cap explants.


Contributors: Ye Jin, Daniel Weinstein

Experiment Type: mRNA profile of pitx1-injected or control animal cap explants at neurula stages were generated by deep sequencing, in duplicate, using Illumina HiSeq2500 by Genewiz.

Experiment Reagents: pitx1.L.

Phenotypes:
pitx1 mRNA + animal cap NF 18

Article: XB-ART-54672, PubMed

Source: NCBI GEO, Xenbase FTP

Samples: (DEG = Differentially Expressed Genes; GSM = GEO Sample Number)
Sample View GSMs Assay Type
animal cap - NF18 GSM3031400  GSM3031402  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
explant   NF stage 9 animal cap
Conditions:
Tissue Assay Stages ChIP Antibody
animal cap NF stage 17 to NF stage 18  
   animal cap - NF18 + pitx1 mRNA DEG GSM3031399  GSM3031401  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection pitx1.L pitx1.L NF stage 3 (4-cell) blastomere
explant   NF stage 9 animal cap
Conditions:
Tissue Assay Stages ChIP Antibody
animal cap NF stage 17 to NF stage 18  
Select All DEG      Select All 
Xtr   
Xla 

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