Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Return to search results

GEO Series: GSE112718

Title: Retinoic acid induced expression of Hnf1β and Fzd4 is required for pancreas development in Xenopus laevis

Summary

Retinoic acid (RA) is required for pancreas specification in Xenopus and other vertebrates. However, the gene network that is directly induced by RA-signalling in this context remains to be defined. We identified the transcription factor Hnf1β and the Wnt-receptor Fzd4/Fzd4s as direct RA-target genes through RNA-sequencing of in vitro generated pancreatic explants. Functional analyses of Hnf1β and Fzd4/Fzd4s in programmed pancreatic explants and whole embryos revealed their requirement for pancreatic progenitor formation and differentiation. Hnf1β and Fzd4/Fzd4s thus appear to be involved in pre-patterning events of the embryonic endoderm allowing for pancreas formation.


Contributors: Gabriela Salinas-Riester, Tomas Pieler, Maja Gere, Claudia Pommerenke, Thomas Lingner

Experiment Type: VegT/Noggin/Cyp26a1 programmed animal cap explants from Xenopus laevis, untreated and treated with RA/CHX and collected 2 hours after RA addition at the equivalent of gastrula stage 11; two replicates A and B for each condition

Experiment Reagents: cycloheximide, cyp26a1, nog,
Retinoic acid, vegt.


Article: XB-ART-54918, PubMed

Source: NCBI GEO, Xenbase FTP

Samples: (DEG = Differentially Expressed Genes; GSM = GEO Sample Number)
Sample View GSMs Assay Type
animal cap explant + nog + vegt + cyp26a1 - NF12.5 GSM3082043  GSM3082044  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection cyp26a1 (Xla) cyp26a1 (Xla) NF stage 2 (2-cell) blastomere
mRNA injection vegt (Xla) vegt (Xla) NF stage 2 (2-cell) blastomere
mRNA injection nog (Xla) nog (Xla) NF stage 2 (2-cell) blastomere
explant   NF stage 8 animal cap
Conditions:
Tissue Assay Stages ChIP Antibody
animal cap NF stage 12.5  
   animal cap explant + nog + vegt + cyp26a1 + Retinoic acid + CHX - NF12.5 DEG GSM3082049  GSM3082050  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection nog (Xla) nog (Xla) NF stage 2 (2-cell) blastomere
mRNA injection cyp26a1 (Xla) cyp26a1 (Xla) NF stage 2 (2-cell) blastomere
mRNA injection vegt (Xla) vegt (Xla) NF stage 2 (2-cell) blastomere
explant   NF stage 8 animal cap
chemical Retinoic acid NF stage 11 animal cap
chemical cycloheximide NF stage 11 animal cap
Conditions:
Tissue Assay Stages ChIP Antibody
animal cap NF stage 12.5  
   animal cap explant + nog + vegt + cyp26a1 + Retinoic acid - NF12.5 DEG GSM3082047  GSM3082048  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection nog (Xla) nog (Xla) NF stage 2 (2-cell) blastomere
mRNA injection cyp26a1 (Xla) cyp26a1 (Xla) NF stage 2 (2-cell) blastomere
mRNA injection vegt (Xla) vegt (Xla) NF stage 2 (2-cell) blastomere
explant   NF stage 8 animal cap
chemical Retinoic acid NF stage 11 animal cap
Conditions:
Tissue Assay Stages ChIP Antibody
animal cap NF stage 12.5  
   animal cap explant + vegt + nog + cyp26a1 + CHX - NF12.5 DEG GSM3082045  GSM3082046  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection nog (Xla) nog (Xla) NF stage 2 (2-cell) blastomere
mRNA injection vegt (Xla) vegt (Xla) NF stage 2 (2-cell) blastomere
mRNA injection cyp26a1 (Xla) cyp26a1 (Xla) NF stage 2 (2-cell) blastomere
explant   NF stage 8 animal cap
chemical cycloheximide NF stage 11 embryo
Conditions:
Tissue Assay Stages ChIP Antibody
animal cap NF stage 12.5  
Select All DEG      Select All 
Xtr   
Xla 

Return to search results